• BayGenomics ES Cell Lines

• Beta Cell Biology Consortium Collection

• Beutler Strains

• Comparative Mouse Genomics Centers Consortium

• Deltagen

• Gene Expression Nervous System Atlas

• Lexicon Pharmaceuticals

• Mammalian Pigmentation

• Mutagenesis for Dev. Defects/Baylor

• Nagy Basic ES Cell Lines

• Sanger Gene Trap ES Cell Lines

• Soriano Gene Trap ES Cell Lines

• UNC Mutagenized ES Cell Lines

• ES Cell Line Information

Sanger Gene Trap ES Cell Lines

Background

The Sanger Institute Gene Trap Resource (SIGTR) mouse ES cell line collection was developed to provide a powerful and cost effective approach using gene trapping to create large numbers of insertional mutations that are immediately accessible to molecular characterization. This resource is based on a new generation of gene trap vector designs that are equipped with site-specific recombination sites for post-insertional modification of the trapped locus to create other desired alleles.

Users of SIGTR ES cell lines are advised to follow the protocols available on the SIGTR website. In addition to individual protocols, all the procedures and protocols can be downloaded from the General Information section of SIGTR's website. Detailed information contained there includes:

• Molecular confirmation of gene trap insertions
• Cell culture media and reagent preparation
• Thawing and growing feeder-independent ES cell lines
• X-gal staining of ES cells
• Sub-cloning gene trap cell lines

MMRRC holdings

The MMRRC holds over 11,800 lines in this collection

Distribution

Cell lines are distributed using the Sanger Institute Gene Trap MTA. Strains are available only to investigators at not-for-profit institutions.

Related Links

• MMRRC catalog of all SIGTR lines
• SIGTR Home page
• SIGTR Search Page
• Sanger Institute Gene Trap MTA
• Parental subclone E14Tg2A.4
• International Gene Trap Consortium
• International Gene Trap Consortium database search
• Images of Feeder Independent Parental ES Cells E14Tg2a.4
• MMRRC's Thawing and Culture protocol of feeder independent E14TG2a.4 cell lines