Soriano Gene Trap ES Cell Lines

Information excerpted from the Soriano website; additional information can be found there.

Background

To identify growth factor regulated genes, gene traps are used in embryonic stem (ES) cells. In this approach, a promoterless reporter gene (for instance encoding beta galactosidase, or beta geo) is introduced into ES cells. Selection for expression of the gene requires transcription from a cellular promoter, and consequently a mutation in a cellular gene, and the activity of the tagged gene can be followed by staining for beta galactosidase activity. Detailed description of methods used for gene trap mutagenesis may be found in Friedrich, G., and Soriano, P. (1993) and Chen, W.V., and Soriano, P. (2003). Large scale sequencing of ES cell clones was conducted and sequence tags have been deposited to NCBI's dbGSS. Sequence searches for genes that have been trapped can be performed by running a BLAST search of the dbGSS database. A list of the genes trapped may be found on the Soriano website.

The recommended citations for the resource are:
Friedrich, G., and Soriano, P., Methods Enzymol. 1993;225:681-701 and
Chen, W.V., and Soriano, P., Methods Enzymol. 2003;365:367-86.

MMRRC holdings

The MMRRC holds over 1,200 cell lines for this resource.

Distribution

Cell lines are distributed using the MMRRC Conditions of Use *(COU). These cell lines are available only to investigators at not-for-profit institutions.

* The preceding link is to the COU text only; the COU user form will be provided after order submission.

Soriano Gene Trap ES Cell Lines

Background

MMRRC holdings

Distribution

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