- MMRRC Major Collections
- BayGenomics ES Cell Lines
- Beta Cell Biology Consortium
- Beutler Mutagenetix
- Comparative Mouse Genomics Centers Consortium
- Consortium for Functional Glycomics
- Deltagen Collection
- Gene Expression Nervous System Atlas
- Genentech Lexicon
- Lexicon Pharmaceuticals
- Mammalian Pigmentation
- Mutagenesis for Dev. Defects/Baylor
- Nagy Basic ES Cell lines
- Neuroscience Blueprint cre
- Pleiades Promoter Project
- Sanger Inst. Gene Trap Resource ES Cell Lines
- Sanger miRNA Knockout ES Cell Lines
- Soriano Gene Trap ES Cell Lines
- UNC Mutagenized ES Cell Lines
- ES Cell Line Information
Soriano Gene Trap ES Cell Lines
Information excerpted from the Soriano website; additional information can be found there.
Background
To identify growth factor regulated genes, gene traps are used in embryonic stem (ES) cells. In this approach, a promoterless reporter gene (for instance encoding beta galactosidase, or beta geo) is introduced into ES cells. Selection for expression of the gene requires transcription from a cellular promoter, and consequently a mutation in a cellular gene, and the activity of the tagged gene can be followed by staining for beta galactosidase activity. Detailed description of methods used for gene trap mutagenesis may be found in Friedrich, G., and Soriano, P. (1993) and Chen, W.V., and Soriano, P. (2003). Large scale sequencing of ES cell clones was conducted and sequence tags have been deposited to NCBI's dbGSS. Sequence searches for genes that have been trapped can be performed by running a BLAST search of the dbGSS database.
The recommended citations for the resource are:
Friedrich, G., and Soriano, P., Methods Enzymol. 1993;225:681-701 and
Chen, W.V., and Soriano, P., Methods Enzymol. 2003;365:367-86.
MMRRC holdings
The MMRRC holds over 1,200 cell lines for this resource. View MMRRC catalog of all Soriano Gene Trap Lines lines.
Distribution
Cell lines are distributed using the MMRRC Conditions of Use *(COU).
* The preceding link is to the COU text only; the COU user form will be provided after order submission.
Related Links
The MMRRC is a collaborative effort, funded by grants from the NIH
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