Strain Detail Sheet

Phenotype:
Homozygous phenotype: Adult male P58^IPK null mice display glucosuria, hyperglycemia, and hypoinsulinemia. P58^IPK null mice show pancreatic cell depletion. Pancreatic cell failure in P58^IPK null mice is associated with increased apoptosis and upregulation of genes associated with apoptosis. P58^IPK null male mice are smaller and can be easily distinguished from heterozygous and wild type mice as early as two weeks of age.

Heterozgyous phenotype: None identified.

Strain Type:

Founder genetic background: C57BL/6

Strain genetic background: C57BL/6

Strain Development:
The contribution of these stem cells to the germline of chimeric mice was assessed by breeding with C57BL/6 females and screening for black offspring. Heterozygous mice were identified by PCR and intercrossed to obtain a Mendelian ratio of homozygous offspring. Routine genotyping for homozygosity was done by PCR using primers specific for an internal targeting deletion sequence (flanking the 59 bp Xmal deletion in the targeted P58^IPK allele) and an external genomic sequence. Mice were 100% C57BL/6 (from a C57BL/6 ES cell line), and were maintained on the C57BL/6 background by alternate generation backcrossing. Tissues and embryonic fibroblasts derived from homozygous, heterozygous, and wild-type littermates were processed and subjected to Western blot analysis. All progeny displayed the expected Mendelian ratio of heterozygous versus homozygous genotypes.

• Apoptosis
• Cancer
• Diabetes/Obesity
• Endocrine Deficiency
• Metabolism
• Models for Human Disease
• Research Tools

Donor: Warren C. Ladiges, DVM, University of Washington

Primary Reference:

Ladiges WC, Knoblaugh SE, Morton JF, Korth MJ, Sopher BL, Baskin CR, MacAuley A, Goodman AG, LeBoeuf RC, Katze MG. Pancreatic beta-cell failure and diabetes in mice with a deletion mutation of the endoplasmic reticulum molecular chaperone gene P58IPK. Diabetes. 2005 Apr;54(4):1074-81. (Medline PMID: 15793246)

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
010323-MU-RESUS	Resuscitation from cryopreserved material	$1,906.00	litter a
010323-MU-SPERM	Cryopreserved spermatozoa	$412.00	aliquot b

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.