|
Allele Symbol: Dnajc3tm8663Wcl Name: DnaJ (Hsp40) homolog, subfamily C, member 3 / targeted mutation 8663, Warren C Ladiges Alteration at locus: Targeted Mutation |
Genetic Alterations:
A mouse C57BL/6 genomic DNA library (Genome Systems, Inc.) in the pBeloBAC11 vector was
screened using a restriction fragment probe corresponding to nucleotides
-100 to +360 of the mouse P58^IPK cDNA. Based upon preliminary Southern
blot analyses, a 15 kb HpaI fragment was subcloned, restriction mapped,
and partially sequenced. This sequence was then assembled into an
eGFP/ -galactosidase/PKG-Neo/pA targeting vector designed to replace
P58^IPK exon 1 containing the translation start site. The targeting
vector was transfected into C57BL/6-derived embryonic stem cells.
Genotype Determination:
ES Cell Line: Not applicable
Phenotype:
Homozygous phenotype: Adult male P58^IPK null mice display glucosuria, hyperglycemia, and hypoinsulinemia.
P58^IPK null mice show pancreatic cell depletion. Pancreatic
cell failure in P58^IPK null mice is associated with increased
apoptosis and upregulation of genes associated with apoptosis. P58^IPK
null male mice are smaller and can be easily distinguished from
heterozygous and wild type mice as early as two weeks of age.
Heterozgyous phenotype: None identified.
Mammalian Phenotype Terms:
assigned by genotype
Strain Type:
Founder genetic background: C57BL/6
Strain genetic background: C57BL/6
Strain Development:
The contribution of these stem cells to the germline of chimeric mice was assessed by breeding
with C57BL/6 females and screening for black offspring. Heterozygous mice were
identified by PCR and intercrossed to obtain a Mendelian ratio of
homozygous offspring. Routine genotyping for homozygosity was done by PCR
using primers specific for an internal targeting deletion sequence
(flanking the 59 bp Xmal deletion in the targeted P58^IPK allele) and an
external genomic sequence. Mice were 100% C57BL/6 (from a C57BL/6 ES cell
line), and were maintained on the C57BL/6 background by alternate
generation backcrossing. Tissues and embryonic fibroblasts derived from
homozygous, heterozygous, and wild-type littermates were processed and
subjected to Western blot analysis. All progeny displayed the expected
Mendelian ratio of heterozygous versus homozygous genotypes.
Donor: Warren C. Ladiges, DVM, University of Washington
Primary Reference:
Ladiges WC, Knoblaugh SE, Morton JF, Korth MJ, Sopher BL, Baskin CR, MacAuley A, Goodman AG, LeBoeuf RC, Katze MG. Pancreatic beta-cell failure and diabetes in mice with a deletion mutation of the endoplasmic reticulum molecular chaperone gene P58IPK. Diabetes. 2005 Apr;54(4):1074-81. (Medline PMID: 15793246)
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
|
|||||||||
|
Distribution Fee/unit (US $) |
Units | Notes | ||||||
|---|---|---|---|---|---|---|---|---|---|
|
|
Litter | Recovered litter1; additional fees for any special requests. | ||||||
|
|
Aliquot | Approximate quantity.2 | ||||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.
MMRRC Contact Information
Home Page Feedback
The MMRRC is a collaborative effort, funded by grants from the National Center for Research Resources,
NIH, DHHS.
Web forms on this site assume JavaScript enabled for Netscape 6.x, Microsoft Internet Explorer 5.5 and later versions; forms will not function as expected with earlier versions or when JavaScript is turned off.
Last Modified: June 23, 2010