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Strain Detail Sheet


Strain Name: C57BL/6J-Myd88lkd/Mmcd

Stock Number: 010473-UCD

Other Names:

Gene Details:

Allele Symbol: Myd88lkd
Name: myeloid differentiation primary response gene 88 / lackadaisical
Alteration at locus: Induced Mutation

Genetic Alterations:
undetermined

Genotype Determination:

ES Cell Line: Not applicable

Strain Description

Phenotype:
Homozygous phenotype: Macrophages show no response to TLR7/8, TLR9 ligands

Heterozygous phenotype: Normal as wild type

Mammalian Phenotype Terms:
      assigned by genotype

Myd88lkd/Myd88lkd
        C57BL/6-Myd88
  • immune system phenotype
  • immune system phenotype (MGI Ref ID J:110094)
    • immunoblot analysis of lysates of homozygous mutant mouse embryonic fibroblasts (MEFs) using antibody to IkappaB demonstrates normal degradation by these cells of IkappaB in response to interleukin 1 (IL-1)
    • abnormal adaptive immunity (MGI Ref ID J:110094)
      • abnormal cell-mediated immunity (MGI Ref ID J:110094)
        • abnormal macrophage physiology (MGI Ref ID J:110094)
          • peritoneal macrophages from homozygous mice exhibit diminished type 1 interferon secretion in response to resiquimod, a TLR7-activating ligand
          • peritoneal macrophages from homozygous mice exhibit diminished type 1 interferon secretion in response to unmethylated CpG DNA, a TLR9-activating ligand
          • immunoblot analysis of lysates of homozygous mutant peritoneal macrophages using antibodies to phosphorylated proteins demonstrates failure of these cells to phosphorylate/activate Ikappa B, JUN N-terminal kinase (JNK) or extracellular signal-related kinase (ERK) in response to the TLR7-activating ligand resiquimod
          • immunoblot analysis of lysates of homozygous mutant peritoneal macrophages using antibodies to phosphorylated proteins demonstrates failure of these cells to phosphorylate/activate Ikappa B, JUN N-terminal kinase (JNK) or extracellular signal-related kinase (ERK) in response to the TLR1/TLR2-activating lipopeptide PAM3CSK4
    • abnormal innate immunity (MGI Ref ID J:110094)
      • abnormal macrophage physiology (MGI Ref ID J:110094)
        • peritoneal macrophages from homozygous mice exhibit diminished type 1 interferon secretion in response to resiquimod, a TLR7-activating ligand
        • peritoneal macrophages from homozygous mice exhibit diminished type 1 interferon secretion in response to unmethylated CpG DNA, a TLR9-activating ligand
        • immunoblot analysis of lysates of homozygous mutant peritoneal macrophages using antibodies to phosphorylated proteins demonstrates failure of these cells to phosphorylate/activate Ikappa B, JUN N-terminal kinase (JNK) or extracellular signal-related kinase (ERK) in response to the TLR7-activating ligand resiquimod
        • immunoblot analysis of lysates of homozygous mutant peritoneal macrophages using antibodies to phosphorylated proteins demonstrates failure of these cells to phosphorylate/activate Ikappa B, JUN N-terminal kinase (JNK) or extracellular signal-related kinase (ERK) in response to the TLR1/TLR2-activating lipopeptide PAM3CSK4

Strain Type:

Founder genetic background: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development:

Research Applications

Strain Origin

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

Zhengfan Jiang, Philippe Georgel, Chenglong Li, Jungwoo Choe, Karine Crozat, Sophie Rutschmann, Xin Du, Tim Bigby, Suzanne Mudd, Sosathya Sovath, Ian A. Wilson, Arthur Olson, and Bruce Beutler. Details of Toll-like receptor:adapter interaction revealed by germline mutagenesis. [Submitted]

For additional information, please see: Mutagenetix, a catalogue of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.

Order Request Information

Availability Level:

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution:

Please complete the MMRRC Conditions of Use (COU) and provide it at the time your order is placed. Note: The preceding link is to the COU text only; the COU user form will be provided after order submission.

Fees: *

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)		 Units Notes
010473-UCD-RESUSLitter recovered from cryo-archive
$2,022.00Non-Profit
 Litter Recovered litter1; additional fees for any special requests.
010473-UCD-SPERMCryo-preserved spermatozoa
$437.00Non-Profit
 Aliquot Approximate quantity.2
010473-UCD-EMBRYOCryo-preserved embryos
$1,038.00Non-Profit
 Aliquot Approximate quantity3: 20-40 embryos / aliquot

Control Mice:

  • Wildtype littermates

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.

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Last Modified: June 23, 2010