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Allele Symbol: Sgcbtm1Kcam Name: targeted mutation 1, Kevin P Campbell Alteration at locus: Knockout |
Genetic Alterations:
A targeting vector replaced exons three to six with phosphoglycerate kinase
promoter/neomycin phosphotransferase cDNA by homologous recombination.
Genotype Determination:
ES Cell Line: R1
Phenotype:
Homozygous phenotype: Only known mutation is replacement of exons three to six with phosphoglycerate
kinase promoter/neomysin phosphotransferase cDNA.
Heterozygous phenotype: Heterzygotes do not show any known morphological abnormalities.
Strain Type:
Founder genetic background: 129xC57BL/6
Strain genetic background: C57BL/6J
Strain Development:
Original mouse on BL6/129 background. Since then they have been backcrossed 5 times onto
C57/BL6.
Donor: Kevin P. Campbell, Ph.D., Howard Hughes Medical Institute
Primary Reference:
Durbeej M, Cohn RD, Hrstka RF, Moore SA, Allamand V, Davidson BL, Williamson RA, Campbell KP. Disruption of the beta-sarcoglycan gene reveals pathogenetic complexity of limb-girdle muscular dystrophy type 2E. Mol Cell. 2000 Jan;5(1):141-51. (Medline PMID: 10678176)
Mice with decreased mobility may benefit from gruel as food/water source.
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 010773-MU-RESUS | Resuscitation from cryopreserved material | $1,963.00 | litter a |
| 010773-MU-SPERM | Cryopreserved spermatozoa | $424.00 | aliquot b |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.
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Last Modified: January 11, 2010