Strain Name: B6;129P2-B3galt5tm1Dgen/Mmnc
Stock Number: 011604-UNC
Other Names:
Gene Details: (provided by MGI)
|
Allele Symbol: B3galt5tm1Dgen Name: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5; targeted mutation 1, Deltagen Alteration at locus: Knockout |
Genetic Alterations:
The endogenous gene promoter drives expression of inserted lacZ gene.
Genotype Determination:
ES Cell Line: E14
Strain Description
Phenotype:
There were no significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice.
Phenotypic data provided by Deltagen, Inc. Full report available through MGI data link (includes genotyping information under "Molecular Biology" at top of screen).
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
B3galt5tm1Dgen/B3galt5tm1Dgen
involves: 129P2/OlaHsd * C57BL/6- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:101679)
- physical examination, necropsy, histological examination of tissues and organs, bone marrow section evaluation, blood counts and differential cell counts, clinical chemistry panels, densitometry, and behavior tests reveal no differences from wildtype
- no abnormal phenotype detected (MGI Ref ID J:101679)
Founder genetic background: 129 P2/B6
Strain genetic background: C57BL/6
Strain Development:
ES cells derived from the 129/OlaHsd mouse substrain were used to generate chimeric mice. F1 mice were generated by breeding with C57BL/6 females. F2 homozygous mutant mice were produced by intercrossing F1 heterozygous males and females. Subsequent crosses made to C57BL/6.
Research Applications
Strain Origin
Donor: Deltagen
Primary Reference:
NIH acquires knockout mice. Nature Genetics. 37:1167 (2005).
Special Considerations
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires execution of the Deltagen Standard Academic license agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|
Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
|
Distribution Fee/unit (US $) |
Units | Notes | ||||
|---|---|---|---|---|---|---|---|
|
|
Litter | Recovered litter1; additional fees for any special requests. | ||||
|
|
Aliquot | Approximate quantity3: 20-40 embryos / aliquot | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
