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Allele Symbol: B3gnt3tm1Dgen Name: targeted mutation 1, Deltagen Alteration at locus: Knockout |
Genetic Alterations:
The endogenous gene promoter drives expression of inserted lacZ gene.
Genotype Determination:
ES Cell Line: E14
Phenotype:
Homozygous mutants displayed a significantly decreased response latency during hot plate testing. There were no other significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice.
Phenotypic data provided by Deltagen, Inc. Full report available through MGI data link (includes genotyping information under "Molecular Biology" at top of screen).
Strain Type: Congenic
Founder genetic background: 129 P2/B6
Strain genetic background: C57BL/6
Strain Development:
ES cells derived from the 129/OlaHsd mouse substrain were used to generate chimeric mice. F1 mice were generated by breeding with C57BL/6 females. The resultant F1N0 heterozygotes were backcrossed to C57BL/6 mice to generate F1N1 heterozygotes. F2N1 homozygous mutant mice were produced by intercrossing F1N1 heterozygous males and females. Subsequent crosses made to C57BL/6.
Donor: Deltagen
Primary Reference:
NIH acquires knockout mice. Nature Genetics. 37:1167 (2005).
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 011606-UNC-RESUS | Resuscitation from cryopreserved material | $1,906.00 | litter a |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). For additional information about this strain, please email Customer Service at service@mmrrc.org or call (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls).
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The MMRRC is a collaborative effort, funded by grants from the National Center for Research Resources,
NIH, DHHS.
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Last Modified: July 17, 2008