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Allele Symbol: Pomt1Gt(OST194127)Lex Name: protein-O-mannosyltransferase 1 / gene trap 205, Lexicon Genetics Alteration at locus: Gene Trap |
Genetic Alterations:
he targeted mouse gene is Pomt1 (protein-O-mannosyltransferase 1), the ortholog of human POMT1. Aliases include RT and homolog of rotated abdomen, Drosophila. Retroviral insertion disrupted the gene between coding exons 2 and 3.
Genotype Determination:
ES Cell Line: Lexicon 129/SvEvBrd
Phenotype:
Phenotypic data provided by Lexicon Genetics, Inc. Full report available through MGI data link (includes genotyping information under "Genetics" on left side of screen).
Strain Type: Non inbred stock.
Founder genetic background: 129/SvEvBrd x C57BL/6J albino
Strain genetic background: 129/SvEvBrd x C57BL6/J mix
Strain Development:
Targeted or gene trap mutations are generated in strain 129/SvEvBrd-derived embryonic stem (ES) cells. The chimeric mice are bred to C57BL/6J albino mice to generate F1 heterozygous animals. These progeny are intercrossed to generate F2 wild type, heterozygous, and homozygous mutant progeny. On rare occasions, for example when very few F1 mice are obtained from the chimera, F1 heterozygous mice are crossed to 129/SvEvBrd x C57BL/6 hybrid mice to yield additional heterozygous animals for the intercross to generate the F2 mice. Level I phenotypic analysis is performed on mice from this generation.
Donor: Lexicon Genetics Incorporated
Primary Reference:
NIH acquires knockout mice. Nature Genetics. 37:1167 (2005).
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 011649-UNC-RESUS | Resuscitation from cryopreserved material | $1,963.00 | litter a |
| 011649-UNC-EMBRYO | Cryopreserved embryo | $1,008.00 | aliquot c |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.
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The MMRRC is a collaborative effort, funded by grants from the National Center for Research Resources,
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Last Modified: January 11, 2010