Strain Detail Sheet

Phenotype:
Genetic data indicate that this retroviral insertion resulted in lethality of the homozygous mutants. No notable phenotype was observed for the heterozygous mice. Due to lethality, transcript expression analysis was not performed.
Phenotypic data provided by Lexicon Genetics, Inc. Full report available through MGI data link (includes genotyping information under "Genetics" on left side of screen).

Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

Slc35a1^{Gt(OST124815)Lex}/Slc35a1^{Gt(OST124815)Lex}

        involves: 129S5/SvEvBrd * C57BL/6J

• mortality/aging
  • complete preweaning lethality (MGI Ref ID J:103485)
    • no homozygotes seen at weaning; stage of lethality not specified

Founder genetic background: 129/SvEvBrd x C57BL/6J albino

Strain genetic background: 129/SvEvBrd x C57BL6/J mix

Strain Development:
Targeted or gene trap mutations are generated in strain 129/SvEvBrd-derived embryonic stem (ES) cells. The chimeric mice are bred to C57BL/6J albino mice to generate F1 heterozygous animals. These progeny are intercrossed to generate F2 wild type, heterozygous, and homozygous mutant progeny. On rare occasions, for example when very few F1 mice are obtained from the chimera, F1 heterozygous mice are crossed to 129/SvEvBrd x C57BL/6 hybrid mice to yield additional heterozygous animals for the intercross to generate the F2 mice. Level I phenotypic analysis is performed on mice from this generation.

Donor: Lexicon Genetics Incorporated

Primary Reference:

NIH acquires knockout mice. Nature Genetics. 37:1167 (2005).

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.) Request this Strain
MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
011671-UNC-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit	Litter	Recovered litter1; additional fees for any special requests.
011671-UNC-EMBRYO Cryo-preserved embryos	$1,038.00 Non-Profit	Aliquot	Approximate quantity3: 20-40 embryos / aliquot

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.