Strain Name: B6;129S5-Olr1tm1Lex/Mmcd
Stock Number: 011748-UCD
Other Names:
Gene Details: (provided by MGI)
|
Allele Symbol: Olr1tm1Lex Name: oxidized low density lipoprotein (lectin-like) receptor 1; targeted mutation 1, Lexicon Genetics Alteration at locus: Knockout |
Genetic Alterations:
The disrupted mouse gene is Olr1 (oxidized low density lipoprotein [lectin-like] receptor 1), ortholog of human OLR1. Aliases included LOX-1; LOX1; SR-EI; Scare1; lectin-type oxidized LDL receptor 1; and scavenger receptor class E, member 1. Coding exon 1 was targeted.
Genotype Determination:
- Donor's PCR
- See "MGI data link" (below) for genotyping information
ES Cell Line: Lexicon 129/SvEvBrd
Strain Description
Phenotype:
No notable phenotype.
Phenotypic data provided by Lexicon Genetics, Inc. Full report available through MGI data link (includes genotyping information under "Genetics" on left side of screen).
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
Olr1tm1Lex/Olr1tm1Lex
involves: 129S5/SvEvBrd * C57BL/6J- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:103485)
Founder genetic background: 129/SvEvBrd x C57BL/6J albino
Strain genetic background: 129/SvEvBrd x C57BL6/J mix
Strain Development:
Targeted or gene trap mutations are generated in strain 129/SvEvBrd-derived embryonic stem (ES) cells. The chimeric mice are bred to C57BL/6J albino mice to generate F1 heterozygous animals. These progeny are intercrossed to generate F2 wild type, heterozygous, and homozygous mutant progeny. On rare occasions, for example when very few F1 mice are obtained from the chimera, F1 heterozygous mice are crossed to 129/SvEvBrd x C57BL/6 hybrid mice to yield additional heterozygous animals for the intercross to generate the F2 mice. Level I phenotypic analysis is performed on mice from this generation.
Research Applications
Strain Origin
Donor: Lexicon Genetics Incorporated
Primary Reference:
NIH acquires knockout mice. Nature Genetics. 37:1167 (2005).
Special Considerations
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires execution of the Lexicon Simple Letter Agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|
Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
|
Distribution Fee/unit (US $) |
Units | Notes | ||||
|---|---|---|---|---|---|---|---|
|
|
Litter | Recovered litter1; additional fees for any special requests. | ||||
|
|
Aliquot | Approximate quantity.2 | ||||
|
|
Aliquot | Approximate quantity3: 20-40 embryos / aliquot | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
