Strain Detail Sheet

Phenotype:
Homozygous phenotype: Mouse macrophages do not respond to diacyl or triacyl lipopeptides or other TLR2-dependent microbial signature molecules. Mice immunocompromised in response to Gram-positive bacterial infections.

Heterozygous phenotype: none

Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

Tlr2^lngd/Tlr2^lngd

        C57BL/6J-Tlr2<lngd>

• immune system phenotype
  • abnormal macrophage physiology (MGI Ref ID J:94077)
    • mutant macrophages do not respond to diacyl or triacyl lipopeptides or other TLR2-dependent microbial signature molecules
  • increased susceptibility to bacterial infection (MGI Ref ID J:94077)
    • mice are immunocompromised in response to Gram-positive bacterial infection

Founder genetic background: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development:
The founder was a G3 mouse in an ENU mutagenesis program, identified as a phenodeviant with no TLR2 responses. The mutation was fixed on the C57BL/6 background.

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Clean environment advised; sterile environment not necessary.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

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MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
012838-UCD-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit	Litter	Recovered litter1; additional fees for any special requests.
012838-UCD-SPERM Cryo-preserved spermatozoa	$437.00 Non-Profit	Aliquot	Approximate quantity.2
012838-UCD-EMBRYO Cryo-preserved embryos	$1,038.00 Non-Profit	Aliquot	Approximate quantity3: 20-40 embryos / aliquot

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.