Strain Detail Sheet

Phenotype:
1. Hypersensitivity to 4-nitroquinoline-1-oxide
2. Reduced replicative potential
3. Reduced expression of the endogenous WRN protein

Strain Type: Congenic

Founder genetic background: C57BL/6 crossed with C3H

Strain genetic background: C57BL/6

Strain Development:

• Cancer
• Models for Human Disease
• Biology of Aging

Donor: Warren C. Ladiges, DVM, University of Washington

Primary Reference:

Wang L, Ogburn CE, Ware CB, Ladiges WC, Youssoufian H, Martin GM, Oshima J. Cellular Werner phenotypes in mice expressing a putative dominant-negative human WRN gene. Genetics. 2000 Jan;154(1):357-62. (Medline PMID: 10628995 )

Coat color: Black

Other:

MMRRC Breeding System: Backcross

Breeding Scheme(s):

• C57BL/6 female x Heterozygous male

Generation: Unknown

Overall Breeding Performance: Excellent

None

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
000130-MU-RESUS	Resuscitation from cryopreserved material	$1,906.00	litter a
000130-MU-SPERM	Cryopreserved spermatozoa	$412.00	aliquot b
000130-MU-EMBRYO	Cryopreserved embryo	$979.00	aliquot c

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

^c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.