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Strain Detail Sheet


Strain Name: Soriano Gene Trap ES cell line S18-8A1

Stock Number: 013368-UCD

Other Names:

Gene Details:

Alteration at locus: Gene Trap

Genetic Alterations:
Disruption caused by insertion of the retroviral gene trap vector ROSAFARY.

Genotype Determination:

Parental ES Cell Line: AK7.1
Mouse strain from which cell line was derived: 129S4/SvJae

Protocols

Strain Origin

Donor: Philippe Soriano, Ph.D., Mount Sinai Medical Center (See Soriano Lab Overview and Soriano Gene Trap Database)

Primary Reference:

Friedrich G, Soriano P.,
Insertional mutagenesis by retroviruses and promoter traps in embryonic stem cells.
Methods Enzymol. 1993;225:681-701.(Medline PMID: 8231879 )

Chen WV, Soriano P.
Gene trap mutagenesis in embryonic stem cells.
Methods Enzymol. 2003;365:367-86. (Medline PMID: 14696359)

For More Information: See IGTC

Special Considerations

Soriano Parental Cell Line AK7
Soriano Feeder Cell Line SNL 76/7 STO

Health Status Report

Contact MMRRC at UC Davis (mmrrc@ucdavis.edu) for health status information for this ES cell line.

Order Request Information

Availability Level:

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Resequencing adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.

Conditions of Distribution:

Please complete the MMRRC Conditions of Use (COU) and provide it at the time your order is placed. Note: The preceding link is to the COU text only; the COU user form will be provided after order submission.

Fees: *

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)		 Units Notes
013368-UCD-CELLSoriano Gene Trap ES cell line S18-8A1
$546.00Non-Profit
$864.00For-Profit
 Vial Without sequence confirmation.*
recommended serviceMutant ES cell line sequence confirmation service
$279.00Non-Profit
$532.00For-Profit
 per cell line Sequence confirmation recommended.*Service fee is in addition to fee for mutant cell line.
available serviceBasic microinjection service
$3,317.00Non-Profit
$6,070.00For-Profit
 per cell line In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed.
available serviceGuaranteed microinjection service
$5,540.00Non-Profit
$8,073.00For-Profit
 per cell line In addition to the per mutant ES cell line fee (see above); at least one 50% or greater chimeric male, or not charged at all.
015891-UCD-CTRLHigh Passage wild type parental clone AK7 suitable for DNA extraction, control purposes, but not for germline transmission
$56.00Non-Profit
$113.00For-Profit
 Vial  
015891-UCD-CELLLow Passage wild type parental clone AK7 suitable for a single electroporation and germline transmission
$546.00Non-Profit
$864.00For-Profit
 Vial  
015891-UCD-CELLUltra Low Passage wild type parental clone AK7 suitable for multiple electroporations and germline transmission
Custom quote; contact mmrrc@ucdavis.eduNon-Profit
Custom quote; contact mmrrc@ucdavis.eduFor-Profit
   Inquire at mmrrc@ucdavis.edu
015892-UCD-CELLInclude vial of feeder line SNL 76/7 STO
$56.00Non-Profit
$113.00For-Profit
 Vial  

* Resequencing of a cell line is an add-on service; cost is in addition to the cost of the clone itself. We recommend you verify the identity of ES cell line by DNA sequencing (see MMRRC / BayGenomics ES Cell Clones for additional information).

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.

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Last Modified: June 23, 2010