MMRRC Logo

Strain Detail Sheet


Strain Name: Soriano Gene Trap ES cell line S18-8A1

Stock Number: 013368-UCD

Other Names:

Gene Details:

Gene Symbol: Gli2
Name: GLI-Kruppel family member GLI2
Alteration at locus: Gene Trap

Genetic Alterations:
Disruption caused by insertion of the retroviral gene trap vector ROSAFARY.

Genotype Determination:

Parental ES Cell Line: AK7.1
Mouse strain from which cell line was derived: 129S4/SvJae

Protocols

Strain Origin

Donor: Philippe Soriano, Ph.D., Fred Hutchinson Cancer Research Center

Primary Reference:

Friedrich G, Soriano P.,
Insertional mutagenesis by retroviruses and promoter traps in embryonic stem cells.
Methods Enzymol. 1993;225:681-701.(Medline PMID: 8231879 )

Chen WV, Soriano P.
Gene trap mutagenesis in embryonic stem cells.
Methods Enzymol. 2003;365:367-86. (Medline PMID: 14696359)

Special Considerations

Soriano Parental Cell Line AK7
Soriano Feeder Cell Line SNL 76/7 STO

Health Status Report

Contact MMRRC at UC Davis (mmrrc@ucdavis.edu) for health status information for this ES cell line.

Order Request Information

Availability Level:

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Resequencing adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.

Conditions of Distribution:

Please complete the MMRRC Conditions of Use form and provide it at the time your order is placed.

Pricing: *

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution
Fee/unit (US $) 		Units
013368-UCD-Cells ES cell line $500.00 2 vials
015891-UCD-Parental Wild-type parental clone line $500.00 aliquot 
013368-UCD-Reseq Cell line sequence confirmation (resequence) $250.00 per clone  1
013368-UCD-Micro Basic Microinjection service $3,035.00 per clone  2

1 Resequencing of a cell line is an add-on service; cost is in addition to the cost of the clone itself. We recommend you verify the identity of ES cell line by DNA sequencing (see MMRRC / BayGenomics ES Cell Clones for additional information).

2 The basic microinjection service fee is in addition to the clone fee and includes one day of injection and live pup birth — chimeras not guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). For additional information about this strain, please email Customer Service at service@mmrrc.org or call (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls).

 

MMRRC Contact Information     Home Page
The MMRRC is a collaborative effort, funded by grants from the National Center for Research Resources, NCRR logo NIH, DHHS.

Web forms on this site assume JavaScript enabled for Netscape 6.x, Microsoft Internet Explorer 5.5 and later versions; forms will not function as expected with earlier versions or when JavaScript is turned off.

Last Modified: April 14, 2008