Strain Detail Sheet

Phenotype:
This inbred strain has at least 2 coat color gene abnormalities. While homozygous for a mutation at the piebald locus (Ednrb^s), the mutation of interest is at the A locus. Mice with the target mutation are yellow to agouti in coat color and develop obesity and diabetes associated with the yellow coat color. The target mutation is lethal in the homozygous state. Wildtype mice are black; since the strain is maintained in the heterozygous state, wildtype controls are produced at each generation.

Strain Type: Inbred Spontaneous Mutation

Founder genetic background: C3H/HeJ

Strain genetic background: YS

Strain Development:
From Wolff & Pitot Genetics 73:109-123, 1973: "The YS strain was started in 1948 when Chase [Herman B.] introduced recessive spotting into a yellow stock from The Jackson Laboratory and then crossed the stock with C57BL mice (Staats 1972). Breeding stock was supplied by Dr. Chase [to GL Wolf at The Institute for Cancer Research (now Fox Chase Cancer Center), Philadelphia] in 1959; the YS/ChWf substrain has been maintained by sibmating since then."

This strain was moved to NCTR in 1972 and maintained by sibmating with forced heterozygosis by GLW. In 1977 it was derived by caesarian-section and introduced into the SPF barrier colony maintained by NCTR. It was transferred to ML Lamoreux (date unavaible) who submitted it to the MMRRC.

• Diabetes and obesity
• Maternal epigenetics
• Pigmentation research

Donor: Dr. ML Lamoreux, Texas A&M University

Primary Reference:

Duhl DM; Vrieling H; Miller KA; Wolff GL; Barsh GS, Neomorphic agouti mutations in obese yellow mice., Nat Genet 1994 Sep;8(1):59-65. (Medline: 95078924)

Wolff GL. Kodell RL. Moore SR. Cooney CA. Maternal epigenetics and methyl supplements affect agouti gene expression in Avy/a mice. FASEB Journal. 12(11):949-57, 1998 Aug. (Medline: 9707167)

Wolff GL. Roberts DW. Galbraith DB. Prenatal determination of obesity, tumor susceptibility, and coat color pattern in viable yellow (Avy/a) mice. The yellow mouse syndrome. Journal of Heredity. 77(3):151-8, 1986 May-Jun. (Medline: 3734404)

Coat color: Yellow (Heterozygous) / Black (Wildtype)

Other:

MMRRC Breeding System: Sib mating

Breeding Scheme(s):

• Heterozygous (Yellow) Female x Wildtype (Black) Male
• Wildtype (Black) Female x Heterozygous (Yellow) Male

Generation: F20+

Overall Breeding Performance: Good. Yellow mice become obese and consequently show a decline in reproduction earlier than black mice. Females with more agouti color have a longer reproductive lifespan compared with yellow females.

Pair breeding is recommended due to large litter sizes. For optimum breeding performance, feed mouse chow that contains 9 percent fat.

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
000138-MU-RESUS	Resuscitation from cryopreserved material	$1,963.00	litter a
000138-MU-SPERM	Cryopreserved spermatozoa	$424.00	aliquot b

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.