Strain Detail Sheet

Phenotype:
Dark-eyed albinism. This mouse is unique among the albino locus mutations in the extent to which the eye pigmentation differs from the coat pigmentation. Eye color is ruby for young; black for adult. Eye pigmentation of the homozygous adult is intense, coat pigmentation in the adult is white (no pigment).

Characteristics of the Locus: The albino (Tyr) locus encodes the rate-limiting enzyme for pigmentation, which is TYROSINASSE. The absolute requirement for functional TYROSINASE to catalyze the formation of melanin pigment is illustrated by the phenotypes of mice that lack this locus as a result of deletion, for example mice heterozygous for two overlapping deletions, MMRRC:009964 and MMRRC:009965 in which the albino locus is absent. The mice also lack all melanin pigment. Vertebrates that lack normal tyrosinase are characterized by reduction or absence of pigmentation in the eyes and/or skin. If the reduction of pigmentation is severe, optic nerve development may be abnormal, resulting in blindness. Reduction of melanogenesis may result from failure of normal tyrosinase function caused by its abnormal interaction with other proteins, from defects in its regulatory region, from absence or defect of the structural gene. The series of natural mutations that affect availability of normal tyrosinase function includes mutants of this type. C57BL/6J-Tyr^c-2J/Tyr^c-2J, available at The Jackson Laboratory, lacks tyrosinase activity as a result of point mutation in the structural region of the gene; Acromelanic (MMRRC:000140) may have a similar defect. Extreme dilution (MMRRC:000141) seems to have nearly normal pigmentation in the eyes, but their hair pigmentation is a uniform gray. In dark-eyed-albino (MMRRC:000139) the eye pigmentation is somewhat reduced, but intense, while the hair is unpigmented. Chinchilla mice(MMRRC:000144) are very dark, nearly black. Two of the mutant alleles, chinchilla mottled (MMRRC:000145) and extreme dilution mottled (MMRRC:000142) result in chimerism of hair and eye pigmentation that has been attributed to a rearrangement of the DNA 5kb upstream of the tyrosinase locus. One of the genes that regulates the activity of TYROSINASE is MITF. And there are functional interactions between TYROSINASE and TYROSINASE-RELATED PROTEIN within the pigment cell.

Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.

Tyr^c-44H/Tyr^c-44H

        involves: 101/H * C3H/HeH

• pigmentation phenotype
  • abnormal coat/hair pigmentation (MGI Ref ID J:12721)
    • absent coat pigmentation (MGI Ref ID J:12721)
      • the coat is white except for traces of pigmented hair in the ano-genital region, noted only in older mice
  • abnormal eye pigmentation (MGI Ref ID J:12721)
    • reduced eye pigmentation (MGI Ref ID J:12721)
      • evident at birth allowing accurate classification of mice where Tyr^c and Tyr^c-44H are segregating
      • at weaning homozygous mice have dark ruby eyes that intensify to nearly black by 3-4 months of age
• vision/eye phenotype
  • abnormal eye pigmentation (MGI Ref ID J:12721)
    • reduced eye pigmentation (MGI Ref ID J:12721)
      • evident at birth allowing accurate classification of mice where Tyr^c and Tyr^c-44H are segregating
      • at weaning homozygous mice have dark ruby eyes that intensify to nearly black by 3-4 months of age
• integument phenotype
  • abnormal coat/hair pigmentation (MGI Ref ID J:12721)
    • absent coat pigmentation (MGI Ref ID J:12721)
      • the coat is white except for traces of pigmented hair in the ano-genital region, noted only in older mice

Founder genetic background: C3H, 101, and NMRI/HanIcof

Strain genetic background: C57BL/6J

Strain Development:
Radiation induced mutation made at MRC Radiobiology Unit, Harwell, England, likely on hybrid C3H x 101 males. Mutation maintained on NMRI/HanIcof until about 1995. Subsequently, backcrossed to C57BL/6J.

Donor: Dr. M. L. Lamoreux, Texas A&M University

Primary Reference:

Schmidt A, Beermann F. Molecular basis of dark-eyed albinism in the mouse. Proc Natl Acad Sci U S A. 1994 May 24;91(11):4756-60. (Medline: 8197131)

Coat color: White

Other:

MMRRC Breeding System: Sib mating

Breeding Scheme(s):

• Homozygous Female x Homozygous Male
• Heterozygous Female x Homozygous Male

Generation: N10+

Overall Breeding Performance: Good

Viability and Fertility	Female	Male
Homozygotes are viable:	Yes	Yes
Homozygotes are fertile:	Yes	Yes
Heterozygotes are fertile:	Yes	Yes
Age Reproductive Decline:	Unknown	Unknown

Feed 9-percent fat diet.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.) Request this Strain
MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
000139-MU-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit $4,109.00 For-Profit	Litter	Recovered litter1; additional fees for any special requests.
000139-MU-SPERM Cryo-preserved spermatozoa	$437.00 Non-Profit $817.00 For-Profit	Aliquot	Approximate quantity.2

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.