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Allele Symbol: DctSlt-lt Name: dopachrome tautomerase / slaty light Chromosome: 14 Alteration at locus: Spontaneous Mutation |
Genetic Alterations:
Point mutation (transition) in gene.
Genotype Determination:
ES Cell Line: Not applicable
Phenotype:
Mice homozygous for the mutant allele have a medium gray hair
coat. Dopachrome tautomerase is one of the three enzymes that
function in the melanogenic pathway.
Strain Type: Congenic Spontaneous Mutation
Founder genetic background: C3H/HeJ
Strain genetic background: C57BL/6
Strain Development:
Spontaneous mutation occurred on the C3H/HeJ. Backcrossed to
C57BL/6J over 10 generations.
Donor: Dr. ML Lamoreux, Texas A&M University
Primary Reference:
Budd PS. Jackson IJ. Structure of the mouse tyrosinase-related protein-2/dopachrome tautomerase (Tyrp2/Dct) gene and sequence of two novel slaty alleles. Genomics. 29(1):35-43, 1995 Sep 1. (Medline PMID: 8530099)
Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31. (Medline PMID:11277491)
Coat color: Medium gray
Other:
MMRRC Breeding System: Sib mating, with occassional backcross to C57BL/6.
Breeding Scheme(s):
Generation: Unknown
Overall Breeding Performance: Good (similar to C57BL/6)
| Viability and Fertility | Female | Male |
|---|---|---|
| Homozygotes are viable: | Yes | Yes |
| Homozygotes are fertile: | Yes | Yes |
| Heterozygotes are fertile: | Yes | Yes |
| Age Reproductive Decline: | 26 weeks | 26 weeks |
Average litter size: Unknown
Recommended wean age: 4 weeks
Maintain on a 9% fat diet. Note wean age.
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 000150-MU-RESUS | Resuscitation from cryopreserved material | $1,963.00 | litter a |
| 000150-MU-SPERM | Cryopreserved spermatozoa | $424.00 | aliquot b |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.
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The MMRRC is a collaborative effort, funded by grants from the National Center for Research Resources,
NIH, DHHS.
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Last Modified: January 11, 2010
