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Allele Symbol: 4921505C17Riktm1.3Mgn Name: RIKEN cDNA 4921505C17 gene / targeted mutation 1.3, Mark A Magnuson Alteration at locus: Knock-In |
Genetic Alterations:
This knock-in
(null) allele resulted from a multi-allelic gene targeting strategy that
involved the use of both FRT and loxP sites. This pia[lacZ] allele
contains a lacZ-pA region flanked on the 5' side by a single (remnant)
FRT site and one the 3' side by a single loxP site.
Genotype Determination:
ES Cell Line: TL-1
Phenotype:
Homozygous phenotype: Homozygous exhibit
an embryonic lethal phenotype. Embryos exhibit growth arrest after E9.5
and die by E11.5.
Heterozygous phenotype: Heterozyous animals are viable and do not exhibit any obvious mutant
phenotype.
Check for additional phenotype data at MMRRC UC Davis
Strain Type:
Founder genetic background: 129S6/SvEvTac
Strain genetic background: 98.5% C57BL/6J + 1.5% 129S6
Strain Development:
A multiallelic gene targeting was
developed that was used to generate a pia[lacZ+neo] allele in mouse
embryonic stem cells. After germline transmission a neoR cassette and
exon 3 (which were flanked by loxP sites) were removed by mating to EIIa-
Cre transgenic mice, thereby generating mice containing the pia[lacZ]
allele. Pia[lacZ} mice were subsequently backcrossed into a C57BL/6J
background.
Donor: Mark A. Magnuson, M.D., Vanderbilt University
Primary Reference:
Shiota C, Woo JT, Lindner J, Shelton KD, Magnuson MA. Multiallelic disruption of the rictor gene in mice reveals that mTOR complex 2 is essential for fetal growth and viability. Dev Cell. 2006 Oct;11(4):583-9. Epub 2006 Sep 7. (Medline PMID: 16962829)
None
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 015200-UCD-Resus | Resuscitation from cryopreserved material | $1,850.00 | litter a |
| 015200-UCD-Sperm | Cryopreserved spermatozoa | $400.00 | aliquot b |
| 015200-UCD-Embryos | Cryopreserved embryos | $950.00 | aliquot c |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). For additional information about this strain, please email Customer Service at service@mmrrc.org or call (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls).
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Last Modified: April 29, 2008