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Strain Detail Sheet


Strain Name: BayGenomics ES Parental cell line E14Tg2a.4

Stock Number: 015890-UCD

Other Names:

Gene Details:

Alteration at locus: Non-mutant

Genetic Alterations:
View MMRRC's Major Collection page for BayGenomics cells.

Genotype Determination:

Parental ES Cell Line: E14Tg2A (feeder free cell line derived from E14TG2a)

Images are of Feeder Independent Parental ES Cells E14Tg2a.4

Mouse strain from which cell line was derived: 129P2/OlaHsd

Protocols

Strain Origin

Donor: BayGenomics

Primary Reference:

Brennan J, Skarnes WC. Gene trapping in mouse embryonic stem cells. Methods Mol Biol. 1999;97:123-38. Review. (Medline PMID:10443362)

Hooper M; Hardy K; Handyside A; Hunter S; Monk M, HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells., Nature 1987 Mar 19-25;326(6110):292-5 (Medline PMID: 3821905 )

Special Considerations

Eye and Coat Color of 129P2/OlaHsd (available from Harlan) is pink-eyed chinchilla.

Health Status Report

Health Status Report

Order Request Information

Availability Level:

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks.

Conditions of Distribution:

Please complete the MMRRC Conditions of Use (COU) and provide it at the time your order is placed. Note: The preceding link is to the COU text only; the COU user form will be provided after order submission.

Fees: *

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)		 Units Notes
015890-UCD-CTRLBayGenomics High Passage Parental cell line E14Tg2a.4 (suitable for DNA extraction, control purposes; not for germline transmission)
$56.00Non-Profit
 Vial Suitable for DNA extraction, control purposes but not for germline transmission.
015890-UCD-CELLBayGenomics Low Passage Parental cell line E14Tg2a.4 (suitable for electroporation and germline transmission)
$546.00Non-Profit
 Vial Suitable for a single electroporation and germline transmission.
015890-UCD-ULTRABayGenomics Ultra-low Passage Parental cell line E14Tg2a.4 (suitable for multiple electroporations and germline transmission)
Custom quote; contact mmrrc@ucdavis.eduNon-Profit
 Vial Suitable for a multiple electroporation and germline transmission.

* Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the clone itself. We recommend verification of the identity of ES cell line by genetic confirmation (see MMRRC ES Cell Clones and Related Cell Lines for additional information).

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

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Last Modified: July 12, 2011