Strain Detail Sheet

Phenotype:
Homozygous phenotype: Homozygous concrete mice display hyperpigmentation, with a gray coat color, and do not express the small GTPase Rab27a, while still expressing detectable amounts of the Rab27a effector JFC1/Slp1. These mice also show an impaired secretion of myeloperoxidase (MPO) into the plasma in response to lipopolysaccharide (LPS).

Heterozygous phenotype: Not applicable
Check for additional phenotype data at MMRRC UC Davis

Strain Type:

Founder genetic background: C57BL/6

Strain genetic background: C57BL/6

Strain Development:
G0 C57BL/6 males are treated with ENU and bred with C57BL/6 females. G1 males are bred with C57BL/6 females, and females from the progeny are backcrossed with G1 males. Concrete arose from the G3 generation.

• Cell Biology
• Immunology and Inflammation
• Models for Human Disease

Donor: Bruce Beutler, Ph.D., The Scripps Research Institute

Primary Reference:

Munafo DB, Johnson JL, Ellis BA, Rutschmann S, Beutler B, Catz SD. Rab27a is a key component of the secretory machinery of azurophilic granules in granulocytes. Biochem J. 2006 Nov 7; [Epub ahead of print] (Medline PMID: 17090228)

poor clotting ability (excessive bleeding)

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
016836-UCD-Resus	Resuscitation from cryopreserved material	$1,850.00	litter a
016836-UCD-Sperm	Cryopreserved spermatozoa	$400.00	aliquot b
016836-UCD-Embryos	Cryopreserved embryos	$950.00	aliquot c

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

^c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.