Strain Detail Sheet

Phenotype:
Homozygous phenotype: None

Hemizygous phenotype: Using IL-21-transgenic mice it has been demonstrated that although IL-21 induces death of resting B cells, it promotes differentiation of B cells into postswitch and plasma cells. Thus, IL-21 differentially influences B cell fate depending on the signaling context, IL-21 can be proapoptotic for B cells in vitro yet critical for Ag-specific Ig production in vivo.

Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.

Tg(H2-K^b-IL21)5Wjl/-

        involves: 129

• immune system phenotype
  • abnormal B cell differentiation (MGI Ref ID J:93740)
    • B cell differentiation is driven towards postswitch cells unlike in wild-type mice
    • abnormal class switch recombination (MGI Ref ID J:93740)
      • mice exhibit an increase in Ig class-switched B cells compared to in wild-type mice
    • decreased transitional stage T2 B cell number (MGI Ref ID J:93740)
    • increased immature B cell number (MGI Ref ID J:93740)
      • in the periphery
      • increased transitional stage T1 B cell number (MGI Ref ID J:93740)
  • decreased mature B cell number (MGI Ref ID J:93740)
    • decreased follicular B cell number (MGI Ref ID J:93740)
    • decreased marginal zone B cell number (MGI Ref ID J:93740)
  • increased B cell apoptosis (MGI Ref ID J:93740)
  • increased IgG1 level (MGI Ref ID J:93740)
  • increased IgM level (MGI Ref ID J:93740)
• hematopoietic system phenotype
  • abnormal B cell differentiation (MGI Ref ID J:93740)
    • B cell differentiation is driven towards postswitch cells unlike in wild-type mice
    • abnormal class switch recombination (MGI Ref ID J:93740)
      • mice exhibit an increase in Ig class-switched B cells compared to in wild-type mice
    • decreased transitional stage T2 B cell number (MGI Ref ID J:93740)
    • increased immature B cell number (MGI Ref ID J:93740)
      • in the periphery
      • increased transitional stage T1 B cell number (MGI Ref ID J:93740)
  • decreased mature B cell number (MGI Ref ID J:93740)
    • decreased follicular B cell number (MGI Ref ID J:93740)
    • decreased marginal zone B cell number (MGI Ref ID J:93740)

Founder genetic background: 129

Strain genetic background: C57BL/6

Strain Development:
No obvious phenotype development. Normal Hemizygous breeding. TG X WT

Donor: Warren Leonard, M.D., NIH

Primary Reference:

Ozaki K, Spolski R, Ettinger R, Kim HP, Wang G, Qi CF, Hwu P, Shaffer DJ, Akilesh S, Roopenian DC, Morse HC 3rd, Lipsky PE, Leonard WJ. Regulation of B cell differentiation and plasma cell generation by IL-21, a novel inducer of Blimp-1 and Bcl-6. J Immunol. 2004 Nov 1;173(9):5361-71. (Medline PMID: 15494482)

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.) Request this Strain
MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
016978-UNC-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit	Litter	Recovered litter1; additional fees for any special requests.
016978-UNC-SPERM Cryo-preserved spermatozoa	$437.00 Non-Profit	Aliquot	Approximate quantity.2

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.