Strain Detail Sheet

Phenotype:
Description of White Spotting mutants
Description of White Kit mutants

A dominant mutation that is lethal in the homozygous state. Mice that carry the mutant allele are black with white spotting; homozygous ^+/+ mice are solid black. Heterozygous mice with the mutant allele have fewer pigment cells (more white areas) than heterozygous mutant mice on the C57BL/J background.

Please note: This strain is identical to MMRRC:000178 in every aspect except that strain 178 has a high incidence of hypopigmention whereas 177 has a "normal" or average level of expression.

In this collection of mice, the identical allele of origin is expressed in five different degrees of severity (stocks 173, 174, 175, 177, 178), depending upon the congenic background, and within the congenic mice upon two (or more) discrete modifying genes that have been maintained by selection. This stock (MMRRC:000177) was created by backcrossing the original mutant, which was obtained from The Jackson Laboratory, where the mutation occurred in the C57BL/6J stocks of Dr. Elizabeth Russell, onto a JU/CtLm background. The allele is homozygous lethal. Heterozygotes exhibit at least twice as much white area as the same allele on the B6 background.

Strain Type: Congenic Spontaneous Mutation

Founder genetic background: C57BL/6J

Strain genetic background: JU/CtLm (MMRRC:000136)

Strain Development:
Spontaneous mutation arose on a C57BL/6J; subsequently backcrossed to JU/CtLm.

• Pigment cell precursors during embryogenesis
• Gene interactions affecting pigmentation
• Embryonic development of mast cells, germ cells or erythrocytes

Donor: Dr. ML Lamoreux, Texas A&M University

Primary Reference:

Lamoreux ML, Russell ES. Developmental interaction in the pigmentary system of mice. I. Interactions between effects of genes on color of pigment and on distribution of pigmentation in the coat of the house mouse (Mus musculus). J Hered. 1979 Jan-Feb;70(1):31-6. (Medline: 469221)

Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Pigment. Cell Res. 1999 Dec;12(6):383-90. (Medline: 10614578)

Mackenzie MA, Jordan SA, Budd PS, Jackson IJ. Activation of the receptor tyrosine kinase Kit is required for the proliferation of melanoblasts in the mouse embryo. Dev Biol. 1997 Dec 1;192(1):99-107. (Medline PMID: 9405100 )

Geissler EN, Ryan MA, Housman DE. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell. 1988 Oct 7;55(1):185-92. (Medline PMID: 2458842 )

Lamoreux ML, Russell ES. Developmental interaction in the pigmentary system of mice. I. Interactions between effects of genes on color of pigment and on distribution of pigmentation in the coat of the house mouse (Mus musculus). J Hered. 1979 Jan-Feb;70(1):31-6. No abstract available. (Medline PMID: 469221 )

Coat color: Heterozygotes are black with white spotting.

Other:

MMRRC Breeding System: Backcross or sib mating

Breeding Scheme(s):

• JU/CtLm female x Heterozygote mutant male
• Heterozygote mutant female x JU/CtLm male
• Heterozygote mutant female x Heterozygote mutant male

Generation: Unknown

Overall Breeding Performance: Excellent

Maintain these mice on a 9% fat breeder chow. Suggest use non-spotted JU/CtLm inbred females each generation if being maintained over a period of time. Kit heterozygous mice (Kit^W-2J/+) have fewer oocytes than normal mice and also the homozygotes are lethal in all cases, so spotted males should be mated to black females in all cases when possible. They should be mated when young. Each of these stocks is best maintained by intercrossing.

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
000177-MU-RESUS	Resuscitation from cryopreserved material	$1,963.00	litter a
000177-MU-SPERM	Cryopreserved spermatozoa	$424.00	aliquot b
000177-MU-EMBRYO	Cryopreserved embryo	$1,008.00	aliquot c

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

^c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.