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Allele Symbol: Ednrbs Name: endothelin receptor type B / piebald Chromosome: 14 Alteration at locus: Spontaneous Mutation |
Genetic Alterations:
This mutation is allelic to a targeted mutation of this gene.
Homozygous mice produce approximately 25% of the normal
levels of transcript from this allele. RT-PCR analysis
demonstrated that no alterations in the coding sequence would
result in any alteration of the amino acid sequence, and
therefore could account for the mutant phenotype.
Genotype Determination:
ES Cell Line: Not applicable
Phenotype:
Heterozygous mice have a white stripe on the head and a prominant white belly spot. Homozygous mice are spotted and prone to the development of megacolon.
Strain Type: Congenic Spontaneous Mutation
Founder genetic background: old mutant of the mouse fancy
Strain genetic background: JU/CtLm (MMRRC:000136)
Strain Development:
Spontaneous mutation arose on "old mutant of the
mouse fancy"; subsequently backcrossed to JU/CtLm (MMRRC:000136).
Donor: Dr. M. L. Lamoreux, Texas A&M University
Primary Reference:
Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Pigment Cell Res. 1999 Dec;12(6):383-90. (Medline: 10614578)
Hosoda K; Hammer RE; Richardson JA; Baynash AG; Cheung JC; Giaid A; Yanagisawa M. Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice. Cell. 1994 Dec 30;79(7):1267-76. (Medline: 8001159)
Dunn LC, Mohr J. An association of hereditary eye defects with white spotting. Proc Natl Acad Sci U S A. 1952 (32): 872-875. (Medline: Not available)
Coat color: Homozygous mice are black and white spotted. Heterozygous mice are black with white stripe on forehead.
Other:
MMRRC Breeding System: Sib mating or backcross
Breeding Scheme(s):
Generation: N10+
Overall Breeding Performance: Excellent
| Viability and Fertility | Female | Male |
|---|---|---|
| Homozygotes are viable: | Poor | Poor |
| Homozygotes are fertile: | Yes | Yes |
| Heterozygotes are fertile: | Yes | Yes |
| Age Reproductive Decline: | Unknown | Unknown |
Average litter size: 10-12
Recommended wean age: 4 weeks
Litters are larger than normal (10-12), so pair breeding is recommended if maintained in small cages. If maintained in larger cages, breeding trios are preferred, as two females care for the large litters better than one female.
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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Distribution Fee/unit (US $) |
Units | Notes | ||||||
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Litter | Recovered litter1; additional fees for any special requests. | ||||||
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Aliquot | Approximate quantity.2 | ||||||
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Aliquot | Approximate quantity3: 20-40 embryos / aliquot | ||||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.
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The MMRRC is a collaborative effort, funded by grants from the National Center for Research Resources,
NIH, DHHS.
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Last Modified: June 23, 2010
