Strain Detail Sheet

Phenotype:
The mutant allele is characterized by yellow color, obesity, and diabetes in heterozygotes. It is prenatal lethal in the homozygous state. Therefore, black offspring will be produced every generation. In mice mutant at the agouti locus, these phenotypes are all reduced if the phenotype of the mouse is more eumelanic than pheomelanic,as is the case when the same mutant allele is backcrossed onto the JU/CtLm (MMRRC:000136) inbred background. The mice are yellow until the first molt, then become umbrous (darker dorsally). After about 6 months of age, this umbrous phenotype is gradually lost.

Mammalian Phenotype Terms:
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.

A^y/A

• adipose tissue phenotype
• increased brown adipose tissue amount (MGI Ref ID J:131039)
• female mice have a 6-fold gain in weight of BAT compared to littermate controls
• increased gonadal fat pad weight (MGI Ref ID J:131039)
• female mice have almost a 7-fold gain in weight of fat pad
• increased inguinal fat pad weight (MGI Ref ID J:131039)
• female mice have a greater than 6-fold gain in weight of fat pad
• increased percent body fat (MGI Ref ID J:131039)
• female mice have almost a 4-fold gain in percentage of body fat compared to littermate controls
• male mice have almost a 1.8-fold gain in percentage of body fat compared to littermate controls
• increased retroperitoneal fat pad weight (MGI Ref ID J:131039)
• female mice have almost a 6-fold gain in weight of fat pad
• behavior/neurological phenotype
• hypoactivity (MGI Ref ID J:131039)
• the locomotor activity of mice is about half that of wild-type mice
• increased eating behavior (MGI Ref ID J:131039)
• 18 week old mice eat about 1.4 times more food during a 7 day period compared to wild-type controls
• growth/size phenotype
• increased body length (MGI Ref ID J:131039)
• female but not male mice have a significant 5% increase in their body length
• increased susceptibility to weight gain (MGI Ref ID J:131039)
• mice gain weight at a greater rate than littermate controls starting at six weeks of age
• obese (MGI Ref ID J:131039)
• by 23 weeks of age, female mice have almost double the weight compared to their wild-type littermate controls
• homeostasis/metabolism phenotype
• increased circulating leptin level (MGI Ref ID J:131039)
• circulating levels of leptin are over 12-fold higher compared to littermate controls
• liver/biliary system phenotype
• increased liver weight (MGI Ref ID J:131039)
• female mice have almost a 2-fold gain in weight of the liver
• pigmentation phenotype
• yellow coat color (MGI Ref ID J:131039)
• mice have a yellow coat color
• skin/coat/nails phenotype
• yellow coat color (MGI Ref ID J:131039)
• mice have a yellow coat color

A^y/a

• Pigmentation
• Diabetes/Obesity
• Reproduction

Donor: Dr. M. L. Lamoreux, Texas A&M University

Primary Reference:

Michaud EJ, Bultman SJ, Klebig ML, van Vugt MJ, Stubbs LJ, Russell LB, Woychik RP. A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (Ay) mutation. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2562-6. (Medline: 8146154)

Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31. (Medline: 11277491)

Ollmann MM, Lamoreux ML, Wilson BD, Barsh GS. Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Genes Dev. 1998 Feb 1;12(3):316-30. (Medline: 9450927)

Coat color: Yellow

Other:

MMRRC Breeding System: Backcross

Breeding Scheme(s):

• JU/CtLm Wildtype Female x Heterozygous Yellow Male
• Heterozygous Yellow Female x JU/CtLm Wildtype Male
• Heterozygous Yellow Female x Heterozygous Yellow Male

Generation: N10+

Overall Breeding Performance: Good for young mice but quickly drops off as mice age. Both males and females quickly become sterile if they become obese.

Feed mouse chow that contains 4 percent fat.

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
000221-MU-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit $4,109.00 For-Profit	Litter	Recovered litter1; additional fees for any special requests.
000221-MU-SPERM Cryo-preserved spermatozoa	$437.00 Non-Profit $817.00 For-Profit	Aliquot	Approximate quantity.2
000221-MU-EMBRYO Cryo-preserved embryos	$1,038.00 Non-Profit $2,621.00 For-Profit	Aliquot	Approximate quantity3: 20-40 embryos / aliquot

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.