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Allele Symbol: Tnfrsf9tm1Byk Name: tumor necrosis factor receptor superfamily, member 9 / targeted mutation 1, Byoung Kwon Alteration at locus: Knockout |
Genetic Alterations:
A neo cassette
was inserted into Nco I site at the initiation codon of Exon II.
Genotype Determination:
ES Cell Line: E13TG2a
Phenotype:
Homozygous phenotype: Reduced NK and NKT
cells in tissues and secondary lymphoid organs under normal condition.
Heterozygous phenotype: None
Check for additional phenotype data at MMRRC UC Davis
Strain Type:
Founder genetic background: C57BL/6J
Strain genetic background: C57BL/6J
Strain Development:
The targeted ES cells were microinjected
into 3- to 5-day-old C57BL/6 blastocysts and transferred to the uterine
horn of pseudopregnant C57BL/6 mice. Eight male and seven female chimeras
were obtained. Donor tested for transmission of the mutated allele by mating
the chimeras with (C57BL/6 x DBA/2)F1 mice. Two males and one female
showed germline transmission of the targeted allele. Homozygous 4-1BB-
deficient mice (4-1BB-/-) were bred with C57BL/6 or BALB/c mice for at
least seven generations to obtain 4-1BB-/- mice on the C57BL/6 or BALB/c
background (-1999).
4-1BB-deficient mice on Balb/c and C57BL/6
background were backcrossed with C57BL/6J or Balb/cJ (Jackson Lab) every
two years in donor's SPF animal facility (since 2000).
Donor: Byoung S. Kwon, Ph.D., University of Ulsan, The Immunomodulation Research Center
Primary Reference:
Kwon BS, Hurtado JC, Lee ZH, Kwack KB, Seo SK, Choi BK, Koller BH, Wolisi G, Broxmeyer HE, Vinay DS. Immune responses in 4-1BB (CD137)-deficient mice. J Immunol. 2002 Jun 1;168(11):5483-90. (Medline PMID: 12023342)
None
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 029589-UCD-Resus | Resuscitation from cryopreserved material | $1,850.00 | litter a |
| 029589-UCD-Sperm | Cryopreserved spermatozoa | $400.00 | aliquot b |
| 029589-UCD-Embryos | Cryopreserved embryos | $950.00 | aliquot c |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). For additional information about this strain, please email Customer Service at service@mmrrc.org or call (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls).
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Last Modified: April 29, 2008