Strain Detail Sheet

Phenotype:
Site specific expression of green fluorescent protein. In situ histochemistry images of the central nervous system of this mouse can be found at St. Jude BGEM. BAC-transgenic images of the central nervous system at various ages of this mouse can be found on the GENSAT project page. Similar images of this mouse can also be found at NCBI.
Check for additional phenotype data MMRRC at UC Davis

Strain Type: Non-inbred

Founder genetic background: FVB/N

Strain genetic background: B6/FVB hybrid

Strain Development:
Modified BAC containing inserted EGFP upstream of targeted gene injected into pronuclei of FVB/N fertilized oocytes. Hemizygous progeny mated to C57BL/6 mice each generation thereafter.

• Cell Biology
• Developmental Biology
• Neurobiology
• Research Tools

Donor: Nathaniel Heintz, Ph.D., The Rockefeller University, GENSAT

Primary Reference:

Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB, Nowak NJ, Joyner A, Leblanc G, Hatten ME, Heintz N. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature. 2003 Oct 30;425(6961):917-25 (Medline PMID: 14586460)

Coat color: White

Other:

MMRRC Breeding System: Outcross to C57BL/6

Breeding Scheme(s):

• 1 C57BL/6 female x 1 Hemizygote male

Generation: N1F2

Overall Breeding Performance: Unknown

Hemizygote transgenics are outcrossed to Wild-Type C57BL/6 mates.

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
030063-UCD-Resus	Resuscitation from cryopreserved material	$1,850.00	litter a
030063-UCD-Sperm	Cryopreserved spermatozoa	$400.00	aliquot b
030063-UCD-Embryos	Cryopreserved embryos	$950.00	aliquot c

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

^c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.