Strain Name: STOCK Myh14tm2Rsad/Mmmh
Stock Number: 030259-MU
Other Names:
- 2CR722C
Gene Details: (provided by MGI)
|
Allele Symbol: Myh14tm2Rsad Name: myosin, heavy polypeptide 14; targeted mutation 2, Robert S. Adelstein Alteration at locus: Knock-In |
Genetic Alterations:
Neo cassette was
inserted into intron flanked by loxP sites. A single amino acid mutation
was introduced by substituting TGC for CGC. Neo cassette was subsequently
removed by crossing mutant mice to CMV-Cre mice.[The wild type allele is CGC and donor changed it to TGC (CGC was replaced by TGC)]
Genotype Determination:
ES Cell Line: CMT-1 129S6SvEv, Millipore Corp.
Strain Description
Phenotype:
Homozygous phenotype: 30% of the
homozygotes develope a lymphoproliferative disease/lymphoma.
Heterozygous phenotype: 30%
of the heterozygotes develope a lymphoproliferative disease/lymphoma.
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Myh14tm2Rsad/Myh14tm2Rsad
involves: 129S/SvEv * C57BL/6- tumorigenesis
- increased lymphoma incidence (MGI Ref ID J:171110)
- 30% of mice develop lymphoproliferative disease with swellings in the neck and abdomen
- increased lymphoma incidence (MGI Ref ID J:171110)
Founder genetic background: 129S6SvEv/C57B6
Strain genetic background: 129S6SvEv/C57B6/BalbC
Strain Development:
129 embryonic stem cells were injected
into C57B6 blastocysts, implanted into C57B6 pseudopregnant mice and
chimeras were born. Male chimeras were mated to C57B6 wild type mice to
generate heterozygotes. Heterozygotes were subsequently crossed to CMV-
Cre mice to remove the neo cassette.
Research Applications
- Apoptosis
- Cancer
- Cell Biology
- Immunology and Inflammation
- Models for Human Disease
- Research Tools
Strain Origin
Donor: Robert S. Adelstein, M.D., NIH
Primary Reference:
Special Considerations
Observe for swelling in the neck and abdomen.
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|
Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
|
Distribution Fee/unit (US $) |
Units | Notes | ||||
|---|---|---|---|---|---|---|---|
|
|
Litter | Recovered litter1; additional fees for any special requests. | ||||
|
|
Aliquot | Approximate quantity.2 | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
