Strain Name: B6;129-Apobec1tm1Rub Ldlrtm1Her Apobtm1Mae/Mmnc
Stock Number: 030638-UNC
Other Names:
- ApoB100beta/100beta
- Apobec1-/-
- LDLR-/-
Gene Details: (provided by MGI)
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Allele Symbol: Apobec1tm1Rub Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1; targeted mutation 1, Edward M Rubin Alteration at locus: Knockout |
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Allele Symbol: Ldlrtm1Her Name: low density lipoprotein receptor; targeted mutation 1, Joachim Herz Alteration at locus: Knockout |
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Allele Symbol: Apobtm1Mae Name: apolipoprotein B; targeted mutation 1, Nobuyo Maeda Alteration at locus: Knockout |
Genetic Alterations:
The apoB100-
allele codes for "VHLTPVEKSAVT" and "KEFTPPVQAAYQ" instead of
"LSVKAQYKKNSD" and "GTSRLMRKRGLK" of the wild-type apoB100 allele at
residues 3143 to 3154 and 3356 to 3366, respectively, in exon 26 of Apob
gene. The two domains of apolipoprotein B are predicted to facilitate
the binding and clearance of LDL particles through the LDL receptor
(LDLR). Replaced amino acid sequences are from the human beta globin.
The precise replacement of approximately 30 bp of each DNA was achieved
by "in" and "out" two-step recombination resulting in duplication and
deletion of the targeted locus. There are no extra DNA fragments
remaining in the mouse genome such as selectable marker genes and loxP
sequences.
Genotype Determination:
ES Cell Line: E14 TG2a
Strain Description
Phenotype:
Homozygous phenotype: ApoB100beta
homozygotes have slightly reduced plasma lipoptrotein levels and are
protected from diet-induced hyperlipidemia. On LDLR-/-, Apobec1-/-
background, homozygotes do not accumulate LDL and are protected from
atherosclerosis.
Heterozygous phenotype: Heterozygotes have an intermediate phenotype.
Founder genetic background: 129 Ola
Strain genetic background: C57BL/6J x 129 mixed
Strain Development:
A mutation in the Apob gene was made in
the ES cells of 129/Ola origin (E14). Mice made from the modified cells
were backcrossed towards C57BL/6J at least 8 generations. Subsequently,
the mutants were crossed with LDLR-/- mice on a C57BL/6J background and
with Apobec1 -/- mice on 129 SvEv (gift from Dr. Eddy Rubin at UC-
Berkley). Resultant triple homozygotes are on mixed genetic backgrounds.
Research Applications
- Cardiovascular
- Metabolism
Strain Origin
Donor: Nobuyo Maeda, Ph.D., University of North Carolina
Primary Reference:
Johnson LA; Altenburg MK; Walzem RL; Scanga LT; Maeda N, Absence of hyperlipidemia in LDL receptor-deficient mice having apolipoprotein B100 without the putative receptor-binding sequences., Arterioscler Thromb Vasc Biol 2008 Oct;28(10):1745-52 (Medline PMID: 18617647)
Toth LR; Smith TJ; Jones C; de Silva HV; Smithies O; Maeda N, Two distinct apolipoprotein B alleles in mice generated by a single 'in-out' targeting., Gene 1996 31;178(1-2):161-8 (Medline PMID: 8921909)
Special Considerations
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Click button to Request this one strain.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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Litter | Recovered litter1; additional fees for any special requests. | ||||
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Aliquot | Approximate quantity.2 | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
