Strain Detail Sheet

Phenotype:
Homozygous phenotype: Rsq2 is X-linked. Heterozygous females are normal, but hemizygous males have abolished TLR7 signaling

Hemizygous phenotype: The rsq2 mutant was discovered in a screen of ENU-mutagenized G3 mice for altered responses to Toll-like receptor (TLR) ligands (TLR Signaling Screen). Peritoneal macrophages from rsq2 mice fail to produce tumor necrosis factor (TNF)-? in response to R-848, a single stranded RNA mimetic that activates TLR7. However, TNF-? production is normal following stimulation with MALP-2 (macrophage-activating lipopeptide-2, TLR2/6 ligand), CpG oligodeoxynucleotides (CpG ODN, TLR9 ligand), Pam3CSK4 (a triacyl lipopeptide, TLR2/1 ligand), poly I:C (TLR3 ligand) and lipopolysaccharide (LPS, TLR4 ligand).

Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

Tlr7^rsq2/-

        C57BL/6J-Tlr7<rsq2>

• immune system phenotype
  • abnormal macrophage physiology (MGI Ref ID J:140020)
    • peritoneal macrophages from hemizygous mutant male mice fail to produce tumor necrosis factor (TNF) alpha in response to R-848, a single stranded RNA analog that signals via TLR7
    • TNF-alpha production by hemizygous mutant macrophages is normal following stimulation with macrophage activating lipopeptide 2 (MALP-2, TLR2/6 ligand), CpG oligodeoxynucleotides (CpG ODN, TLR9 ligand), Pam3CSK4 (a triacyl lipopeptide, TLR1/2 ligand), poly I:C (TLR3 ligand) or lipopolysaccharide (LPS, TLR4 ligand)
  • decreased tumor necrosis factor secretion (MGI Ref ID J:140020)
    • peritoneal macrophages from hemizygous mutant male mice fail to produce tumor necrosis factor (TNF) alpha in response to R-848, a single stranded RNA analog that signals via TLR7
    • TNF-alpha production by hemizygous mutant macrophages is normal following stimulation with macrophage activating lipopeptide 2 (MALP-2, TLR2/6 ligand), CpG oligodeoxynucleotides (CpG ODN, TLR9 ligand), Pam3CSK4 (a triacyl lipopeptide, TLR1/2 ligand), poly I:C (TLR3 ligand) or lipopolysaccharide (LPS, TLR4 ligand)

Founder genetic background: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development:
The rsq2 mutation was generated on a C57BL/6J background. The strain has been maintained on this background.

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

Owen M. Siggs, Nora G. Smart, Bruce Beutler. Record for “rsq2”, updated October 20th, 2008. MUTAGENETIX (TM), B. Beutler and colleagues, Department of Genetics, The Scripps Research Institute, La Jolla, CA.

TLR7 signaling is abolished. The mice will probably be sensitive to certain infections but are healthy under normal conditions.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

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MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
030753-UCD-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit	Litter	Recovered litter1; additional fees for any special requests.
030753-UCD-SPERM Cryo-preserved spermatozoa	$437.00 Non-Profit	Aliquot	Approximate quantity.2
030753-UCD-EMBRYO Cryo-preserved embryos	$1,038.00 Non-Profit	Aliquot	Approximate quantity3: 20-40 embryos / aliquot

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.