Strain Detail Sheet

Phenotype:
Homozygous phenotype: Rsq2 is X-linked. Heterozygous females are normal, but hemizygous males have abolished TLR7 signaling

Hemizygous phenotype: The rsq2 mutant was discovered in a screen of ENU-mutagenized G3 mice for altered responses to Toll-like receptor (TLR) ligands (TLR Signaling Screen). Peritoneal macrophages from rsq2 mice fail to produce tumor necrosis factor (TNF)-? in response to R-848, a single stranded RNA mimetic that activates TLR7. However, TNF-? production is normal following stimulation with MALP-2 (macrophage-activating lipopeptide-2, TLR2/6 ligand), CpG oligodeoxynucleotides (CpG ODN, TLR9 ligand), Pam3CSK4 (a triacyl lipopeptide, TLR2/1 ligand), poly I:C (TLR3 ligand) and lipopolysaccharide (LPS, TLR4 ligand).

Strain Type:

Founder genetic background: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development:
The rsq2 mutation was generated on a C57BL/6J background. The strain has been maintained on this background.

• Immunology and Inflammation

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

For additional information, please see: Mutagenetix, a catalogue of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

TLR7 signaling is abolished. The mice will probably be sensitive to certain infections but are healthy under normal conditions.

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
030753-UCD-RESUS	Resuscitation from cryopreserved material	$1,963.00	per litter a
030753-UCD-SPERM	Cryopreserved spermatozoa	$424.00	per aliquot b
030753-UCD-EMBRYO	Cryopreserved embryo	$1,008.00	per aliquot c

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

^c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.