Strain Name: STOCK Tg(Drd1a-cre)FK164Gsat/Mmcd
Stock Number: 030781-UCD
Other Names:
Gene Details: (provided by MGI)
|
Promoter: Drd1a Name: dopamine receptor D1A Alteration at locus: Transgenic |
|
Transgene: Tg(Drd1a-cre)FK164Gsat Name: transgene insertion FK164, GENSAT Project at Rockefeller University Alteration at locus: Transgenic |
|
Transgene: cre (Bacteriophage P1) Name: cre recombinase Alteration at locus: Transgenic |
Genetic Alterations:
BAC engineering was used to insert an intron containing cre cassettes, followed by a polyadenylation sequence to terminate transcription of the fusion transcript immediately after the recombinase gene, into the BAC vector at the initiating ATG codon in the first coding exon of the gene.
Genotype Determination:
ES Cell Line: Not applicable
Strain Description
Phenotype:
In situ histochemistry images of the central nervous system of this mouse can be found at St. Jude BGEM. BAC-transgenic images of the central nervous system at various ages of this mouse can be found on the GENSAT project page.
Founder genetic background: FVB/N
Strain genetic background: mix of B6/129/Swiss/FVB stocks
Strain Development:
The mice were created in the GENSAT project in collaboration with the Intramural Program of the National Institute of Mental Health. The transgenic mice have a modified BAC-cre injected into pronuclei of FVB/N fertilized oocytes. Hemizygous progeny were mated to non-inbred mice from a mix of B6/129/Swiss/FVB stocks. Mice were sent to NIH/NIMH for testing and NIH/NIMH crossed these with ROSA26-GFP mice to get the desired expression for testing. However, the MMRRC will breed all these strains to C57BL/6J and select for mice with only the cre expression, eliminating the ROSA26-GFP from each line. Mice distributed from these strains will only have cre, and not EGFP.
Research Applications
- Cell Biology
- Developmental Biology
- Neurobiology
- Research Tools
Strain Origin
Donor:
Nathaniel Heintz, Ph.D., The Rockefeller University, GENSAT
Charles Gerfen, Ph.D., National Institutes of Health, National Institute of Mental Health
Primary Reference:
Gong S, Doughty M, Harbaugh CR, Cummins A, Hatten ME, Heintz N, Gerfen CR. Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs. J Neurosci. 2007 Sep 12;27(37):9817-23. No abstract available. (Medline PMID: 17855595)
Colony and Husbandry Information
Appearance
Coat color: Agouti, Black or White
Other:
Breeding
MMRRC Breeding System: Outcross to C57BL/6J
Breeding Scheme(s):
- C57BL/6J female x Hemizygote male
Generation: N2F2
Overall Breeding Performance: Unknown
Reproductive Statistics
| Viability and Fertility | Female | Male |
|---|---|---|
| Homozygotes are viable: | Unknown | Unknown |
| Homozygotes are fertile: | Unknown | Unknown |
| Hemizygotes are fertile: | Unknown | Yes |
| Age Reproductive Decline: | Unknown | Unknown |
Average litter size: 8
Recommended wean age: 3-4 weeks
Special Considerations
None
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|
Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
|
Distribution Fee/unit (US $) |
Units | Notes | ||||
|---|---|---|---|---|---|---|---|
|
|
Litter | Recovered litter1; additional fees for any special requests. | ||||
|
|
Aliquot | Approximate quantity.2 | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
