Strain Detail Sheet

Phenotype:
Site specific expression of green fluorescent protein. In situ histochemistry images of the central nervous system of this mouse can be found at St. Jude BGEM. BAC-transgenic images of the central nervous system at various ages of this mouse can be found on the GENSAT project page. Similar images of this mouse can also be found at NCBI.

Founder genetic background: FVB/N

Strain genetic background: FVB/N-Crl:CD1(ICR) hybrid

Strain Development:
Modified BAC containing inserted EGFP upstream of targeted gene injected into pronuclei of FVB/N fertilized oocytes. Hemizygous progeny mated to Crl:CD1(ICR) mice each generation thereafter.

Donor: Nathaniel Heintz, Ph.D., The Rockefeller University, GENSAT

Primary Reference:

Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB, Nowak NJ, Joyner A, Leblanc G, Hatten ME, Heintz N. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature. 2003 Oct 30;425(6961):917-25 (Medline PMID: 14586460)

Coat color: White

Other:

MMRRC Breeding System: Outcross to Crl:CD1(ICR)

Breeding Scheme(s):

• Crl:CD1(ICR) female x Hemizygous male

Generation: N1F2

Overall Breeding Performance: Unknown

Hemizygous transgenics are outcrossed to wild-type Crl:CD1(ICR) mates.

In general, GENSAT BAC transgenic mouse lines should be utilized in the hemizygous state. Breeding of the BAC transgene to a homozygous state carries an increased risk of generating an unwanted phenotype for two reasons. First, the transgene inserts at random into the genome. Breeding the transgene to homozygosity results in mice that carry this insertion on both chromosomes, increasing the probability that the insertion will result in an observable phenotype. Second, BAC transgenes may carry passenger genes that are also expressed from the BAC vector. Breeding to homozygosity doubles the number of these genes carried in the transgenic mouse, increasing the possibility that a phenotype will result as a consequence of increased gene dosage. In our experience, observable phenotypes are rare in BAC transgenic mice when carried in the hemizygous state.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.