Strain Name: B6.129S1-Mei1m1Jcs/Mmnc
Stock Number: 031721-UNC
Other Names:
- Mei1
Gene Details: (provided by MGI)
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Allele Symbol: Mei1m1Jcs Name: meiosis defective 1 Alteration at locus: Chemically Induced |
Genetic Alterations:
The molecular
defect is an A-to-T transversion at the AG splice acceptor sequence of
intron 11. Two aberrantly spliced transcripts are expressed from this
allele, both resulting in a frameshift of the wild-type sequence and a
predicted truncated protein.
Genotype Determination:
ES Cell Line:
Strain Description
Phenotype:
Homozygous phenotype: Male and female
infertility due to meiotic arrest in the zygotene stage of meiosis I.
These mice are defective in formation of genetically-programmed double
strand breaks, which blocks recombination and syapsis from occurring
properly.
Heterozygous phenotype:
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Mei1m1Jcs/Mei1m1Jcs
involves: 129S1/Sv * C57BL/6J- reproductive system phenotype
- abnormal female meiosis (MGI Ref ID J:60747)
- meiotic defects
- arrest of male meiosis (MGI Ref ID J:60747)
- spermatocytes arrest at zygotene stage of meiosis I
- azoospermia (MGI Ref ID J:60747)
- absent sperm, absence of post-meiotic cells
- female infertility (MGI Ref ID J:60747)
- male infertility (MGI Ref ID J:60747)
- small testis (MGI Ref ID J:60747)
- abnormal female meiosis (MGI Ref ID J:60747)
- endocrine/exocrine gland phenotype
- small testis (MGI Ref ID J:60747)
Founder genetic background: 129S1/SvImJ
Strain genetic background: C57BL/6J
Strain Development:
See MGI:2176973. The mutant line was
derived from EMS mutagenesis of CJ7 ES cells. Over the years, donor backcrossed
to C57BL/6J. At steps along the way, intercrosses were conducted, but derivatives
have been crossed to B6 for the current stocks.
Research Applications
- Developmental Biology
- Reproduction
Strain Origin
Donor: John Schimenti, Ph.D., Cornell University Coll. Veterinary Medicine
Primary Reference:
Libby BJ; Reinholdt LG; Schimenti JC, Positional cloning and characterization of Mei1, a vertebrate-specific gene required for normal meiotic chromosome synapsis in mice., Proc Natl Acad Sci U S A 2003 Dec 23;100(26):15706-11 (Medline PMID: 14668445)
Libby BJ; De La Fuente R; O'Brien MJ; Wigglesworth K; Cobb J; Inselman A; Eaker S; Handel MA; Eppig JJ; Schimenti JC, The mouse meiotic mutation mei1 disrupts chromosome synapsis with sexually dimorphic consequences for meiotic progression., Dev Biol 2002 Feb 15;242(2):174-87 (Medline PMID: 11820814)
Munroe RJ, Bergstrom RA, Zheng QY, Libby B, Smith R, John SW, Schimenti KJ, Browning VL, Schimenti JC. Mouse mutants from chemically mutagenized embryonic stem cells. Nat Genet. 2000 Mar;24(3):318-21. (Medline PMID: 10700192 )
Special Considerations
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||||
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Litter | Recovered litter1; additional fees for any special requests. | ||||||
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Aliquot | Approximate quantity.2 | ||||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
