Strain Name: B6.129X1-Kcnj9tm1Kwn/Mmcd
Stock Number: 031759-UCD
Other Names:
- KCNJ9null
- Kir3.3null
- GIRK3-/-
Gene Details: (provided by MGI)
|
Allele Symbol: Kcnj9tm1Kwn Name: potassium inwardly-rectifying channel, subfamily J, member 9; targeted mutation 1, Kevin Wickman Alteration at locus: Knockout |
Genetic Alterations:
Generation of
null copy of the mouse
KCNJ9 or Kir3.3 or GIRK3 knock out mice-
Kir3.3
knock-out mice. A Cre recombinase-based Kir3.3
targeting strategy was
designed with the goal of obtaining a parental mouse embryonic stem (ES)
cell line that could be further modified to
yield ES cell clonal
derivatives harboring either a
Kir3.3 gene or a “floxed” version of the
mouse Kir3.3 gene. Cloning and characterization of the mouse Kir3.3 gene
has been reported previously
(Wickman et al., 2002).
Genotype Determination:
ES Cell Line: 129/svj
Strain Description
Phenotype:
Homozygous phenotype: no distinguishable
phenotypes
Heterozygous phenotype: no distinguishable
traits
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Kcnj9tm1Kwn/Kcnj9tm1Kwn
involves: 129X1/SvJ- nervous system phenotype
- abnormal synaptic transmission (MGI Ref ID J:77047)
- resting membrane potentials of locus ceruleus neurons in brain slices are depolarized by 15-20 mV relative to neurons from wild-type mice
- abnormal synaptic transmission (MGI Ref ID J:77047)
- behavior/neurological phenotype
- behavior/neurological phenotype (MGI Ref ID J:77047)
- mice are fertile and of normal size, exhibit normal coordination and show no abnormal feeding behavior, grooming, or responses to visual or auditory cues
- no observed propensity for spontaneous and lethal seizures
- behavior/neurological phenotype (MGI Ref ID J:77047)
Founder genetic background: 129 based ESL crossed to C57/BL6
Strain genetic background: C57BL/6
Strain Development:
Research Applications
- Cell Biology
- Metabolism-
- Neurobiology
- Research Tools
- Sensorineural
Strain Origin
Donor: Kevin Wickman, Ph.D., University of Minnesota
Primary Reference:
Torrecilla M, Marker CL, Cintora SC, Stoffel M, Williams JT, Wickman K. G-protein-gated potassium channels containing Kir3.2 and Kir3.3 subunits mediate the acute inhibitory effects of opioids on locus ceruleus neurons. J Neurosci. 2002 Jun 1;22(11):4328-34. (Medline PMID: 12040038 )
Special Considerations
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|
Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
|
Distribution Fee/unit (US $) |
Units | Notes | ||||
|---|---|---|---|---|---|---|---|
|
|
Litter | Recovered litter1; additional fees for any special requests. | ||||
|
|
Aliquot | Approximate quantity.2 | ||||
|
|
Aliquot | Approximate quantity3: 20-40 embryos / aliquot | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
