Strain Name: B6;FVB-Tg(tetO-GCaMP2)12JRyu/Mmmh
Stock Number: 031876-MU
Other Names:
- tet-G2
Gene Details: (provided by MGI)
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Promoter: tetO Name: tetracycline operon Alteration at locus: Transgenic |
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Reporter: GCaMP2 Name: GFP based calcium-sensing molecule Alteration at locus: Transgenic |
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Transgene: Tg(tetO-GCaMP2)12JRyu Name: transgene insertion 12J, Ron Yu Alteration at locus: Transgenic |
Genetic Alterations:
The transgene is
a GFP based Ca sensor GCAMP2 under the control of tetracycline promoter
(tetO).
Genotype Determination:
ES Cell Line:
Strain Description
Phenotype:
Homozygous phenotype: This transgenic
line can be used with any lines expressing tTA or rtTA to activate GCAMP2
expression in the tTA/rtTA cell population. The Ca changes in these cells
can be monitored with fluorescent or multi-photon imaging technique.
Hemizygous phenotype:
Founder genetic background: FVB/N
Strain genetic background: C57BL/6
Strain Development:
The strain is generated by standard
proneuclei injection and has been crossed to C57/B6 mice when possible to
maintain the line.
Research Applications
- Cell Biology
- Neurobiology
- Research Tools
- Sensorineural
Strain Origin
Donor: Ron Yu, Ph.D., Stowers Institute for Medical Research
Primary Reference:
He J, Ma L, Kim S, Nakai J, Yu CR. Encoding gender and individual information in the mouse vomeronasal organ. Science. 2008 Apr 25;320(5875):535-8. (Medline PMID: 18436787)
Special Considerations
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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Litter | Recovered litter1; additional fees for any special requests. | ||||
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Aliquot | Approximate quantity.2 | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
