Strain Name: STOCK Psap tm1Mhor/Mmmh
Stock Number: 032232-MU
Other Names:
- 3A2neo
Gene Details: (provided by MGI)
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Allele Symbol: Psaptm1Mhor Name: prosaposin; targeted mutation 1, Mia Horowitz Alteration at locus: Targeted Mutation |
Genetic Alterations:
A neo cassette
flanked by two loxP sites was inserted between intron 7 and intron 8,
deleting exon 8 of the mouse prosaposin gene.(Construction of the
prosaposin gene targeting vector: A 4.5-kb HindIII-EcoRI fragment,
spanning introns 3 to 7 (including exons 4 to 7) of the prosaposin gene,
and a 2-kb Ecl136II (SacI) fragment, spanning introns 8 to 10 (including
exons 9 and 10) of prosaposin, were isolated from a {lambda}-FIX II mouse
genomic clone (originating from a 129/SvEv genomic library; Stratagene.
HindIII-EcoRI and Ecl136II fragments were subcloned into pET-28 plasmids
(Novagen) and were inserted as the 5' and 3' arms, respectively, in the
loxP/PGK-neo/PGK-tk vector, in which the phosphoglycerate kinase-neomycin
(PGK-neo) cassette is flanked by two loxP sites . The two arms were
cloned on either side of the loxP/PGK-neo cassette, with the neo gene in
the same transcriptional orientation as prosaposin. The 2-kb Ecl136II
fragment was inserted into a Bsu15I blunt-ended site, and the 4.5-kb
HindIII-EcoRI blunt-ended fragment was inserted into a blunt-ended BamHI
site, yielding the targeting vector pPro{Delta}8neoKSloxpPNT, missing the
last 360 bp of intron 7, exon 8, and the first 165 bp of intron 8.)
Genotype Determination:
ES Cell Line: W4 (from Dr Alexandra Joyner)
Strain Description
Phenotype:
Homozygous phenotype: The exon 8
prosaposin mutant mice are healthy and fertile with no obvious phenotype.
No changes were detected in prosaposin secretion or in accumulation and
metabolism of gangliosides, sulfatides, neutral glycosphingolipids,
neutral phospholipids, other neutral lipids, and ceramide. These data
strongly indicate that the prosaposin variant containing the exon 8-
encoded three amino acids is dispensable for normal mouse development and
fertility as well as for prosaposin secretion and its lysosomal function,
at least in the presence of the prosaposin variant missing the exon 8-
encoded three amino acids.
Heterozygous phenotype:
Mammalian Phenotype Terms:(provided by MGI)
Extend all MPTsassigned by genotype
The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Psaptm1Mhor/Psaptm1Mhor
either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss)- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:97577)
- homozygotes are healthy and fertile with normal lipid metabolism
- no abnormal phenotype detected (MGI Ref ID J:97577)
Founder genetic background: 129/SvEv
Strain genetic background: ICR
Strain Development:
Gene-targeted 129/SvEv embryonic stem
(ES) cells were produced by electroporating XhoI-linearized pPro?
8neoKSloxpPNT into W4 (ES) cells, followed by selection with G418 and
gancyclovir. Two hundred fifty double-resistant colonies were generated
and examined by Southern blot analysis. Five properly targeted ES cell
lines were identified, and three were used to produce chimeric males by
morula aggregation. Chimeras were crossed with either 129/SvEv or Swiss
Webster (SW) females to produce heterozygous Pro?8neo mice.
Research Applications
- Models for Human Disease
Strain Origin
Donor: Mia Horowitz Mia, Ph.D., Tel Aviv University
Primary Reference:
Cohen T, Auerbach W, Ravid L, Bodennec J, Fein A, Futerman AH, Joyner AL, Horowitz M. The exon 8-containing prosaposin gene splice variant is dispensable for mouse development, lysosomal function, and secretion. Mol Cell Biol. 2005 Mar;25(6):2431-40. (Medline PMID: 15743835)
Special Considerations
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on the resuscitated animals.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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Litter | Recovered litter1; additional fees for any special requests. | ||||
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Aliquot | Approximate quantity.2 | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (International calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
