Strain Detail Sheet

Phenotype:
Description of White Spotting mutants
Description of Steel Locus mutants

Homozygous mice are black-eyed white; heterozygous mice are black with interspersed white hairs, a very pale belly with a white spot, and a head spot. While homozygotes on the C57BL/6J background die before or shortly after birth, on this background they occasionally survive; surviving homozygous males are fertile.

Familiarly known as Steel-Dickie, this allele was obtained from The Jackson Laboratory, where it occurred and is maintained on a C57BL/6J background. It was used in a study of the impact of the background genome on the size and location of white spots in spotted mice. The belly spots were measured to compare inbred Kitl^Sl-D/+ mice of the JU/CtLm and the C57BL/6J strains. The spots on the JU/CtLm mice were found to be larger and more anterior than those on the C57BL/6J mice, and the JU/CtLm mice had head spots that were lacking in C57BL/6J mice.

Strain Type: Congenic Spontaneous Mutant

Founder genetic background: DBA/2J and BDF1

Strain genetic background: JU/CtLm (MMRRC:000136)

Strain Development:
A C57BL/6J-Kitl^Sl-d male mouse was obtained from The Jackson Laboratory. The allele was backcrossed on to JU/CtLm for at least ten generations (and also maintained on a C57BL/6J background). The ventral spotting on the JU/CtLm mouse is located more anteriorly than is the case with the C57BL mouse. The JU/CtLm mouse has a prominent head spot, while the C57BL/6J mouse does not.

• Hematopoeisis
• Pigmentation
• Mast cell differentiation
• Reproduction
• Modifier genes
• Neural Crest Function
• Cell Survival
• Anemia
• Deafness
• Brain Development
• Mast Cells
• T Cells
• Reproductive Cells
• Cell Surface Receptor/Ligand Interactions
• Cell Migration Patterns

Donor: Dr. ML Lamoreux, Texas A&M University

Primary Reference:

Steel KP, Davidson DR, Jackson IJ. TRP-2/DT, a new early melanoblast marker, shows that steel growth factor (c-kit ligand) is a survival factor., Development 1992 Aug;115(4):1111-9. (Medline PMID: 1280558)

Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Lamoreux, ML. Pigment cell research 12(6):383-90, 1999. (Medline PMID: 10614578)

Rhim H, Dunn KJ, Aronzon A, Mac S, Cheng M, Lamoreux ML, Tilghman SM, Pavan WJ. Spatially restricted hypopigmentation associated with an Ednrbs-modifying locus on mouse chromosome 10. Genome Res. 2000 Jan;10(1):17-29.(Medline PMID: 10645946)

Coat color: The homozygote is white and the heterozygote is black with interspersed white hairs, a very pale belly with a white spot, and a head spot.

Other:

MMRRC Breeding System: Sib mating

Breeding Scheme(s):

• Heterozygous female x Heterozygous male
• Heterozygous mice to black littermates or backcross to MMRRC:000136

Generation: N10+

Overall Breeding Performance: Good in heterozygotes

Feed breeders 9% fat diet.

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
000359-MU-RESUS	Resuscitation from cryopreserved material	$1,963.00	litter a
000359-MU-SPERM	Cryopreserved spermatozoa	$424.00	aliquot b
000359-MU-EMBRYO	Cryopreserved embryo	$1,008.00	aliquot c

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

^c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.