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Allele Symbol: B4galnt1tm1Rlp Name: beta-1,4-N-acetyl-galactosaminyl transferase 1 / targeted mutation 1, Richard L Proia Alteration at locus: Knockout |
Genetic Alterations:
Insertion of neo cassette with deletion of exons 6,7 and part of 8
Genotype Determination:
ES Cell Line: TC-1
Phenotype:
Dysmyelination
Strain Type: Mixed Inbred Targeted Mutation/Knockout
Founder genetic background: B6;129S
Strain genetic background: Mixed Inbred B6;129
Strain Development:
ES cells were injected into C57BL/6; founder offspring were selected and bred to homozygosity.
Donor: Dr. Richard Proia, NIDDK, NIH, DHHS
Primary Reference:
Liu Y, Wada R, Kawai H, Sango K, Deng C, Tai T, McDonald MP, Araujo K, Crawley JN, Bierfreund U, Sandhoff K, Suzuki K, Proia RL. A genetic model of substrate deprivation therapy for a glycosphingolipid storage disorder. J Clin Invest. 1999 Feb;103(4):497-505. (Medline: 10021458)
Coat color: Variable
Other:
MMRRC Breeding System: Sib Mating
Breeding Scheme(s):
Generation: Unknown
Overall Breeding Performance: Unknown
| Viability and Fertility | Female | Male |
|---|---|---|
| Homozygotes are viable: | Yes | Yes |
| Homozygotes are fertile: | Yes | Yes |
| Heterozygotes are fertile: | Yes | Yes |
| Age Reproductive Decline: | Unknown | Unknown |
Average litter size: Unknown
Recommended wean age: 3 weeks
None
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 000036-MU-RESUS | Resuscitation from cryopreserved material | $1,963.00 | litter a |
| 000036-MU-SPERM | Cryopreserved spermatozoa | $424.00 | aliquot b |
| 000036-MU-EMBRYO | Cryopreserved embryo | $1,008.00 | aliquot c |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.
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NIH, DHHS.
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Last Modified: January 11, 2010