Strain Detail Sheet

Phenotype:
Hemizygous mice exhibit ubiquitous expression of GFP in embryos and adult mice.

Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

Nup210l^{Tg(Gt(ROSA)26Sor-EGFP)130910Eps}/Nup210l^{Tg(Gt(ROSA)26Sor-EGFP)130910Eps}

        FVB/NTac-Nup210l<Tg(Gt(ROSA)26Sor-EGFP)130910Eps>/Mmmh

• reproductive system phenotype
  • reproductive system phenotype (MGI Ref ID J:171266)
    • female mice exhibit normal reproductive morphology and fertility
    • asthenozoospermia (MGI Ref ID J:171266)
      • at 8 to 10 weeks of age, sperm motility is reduced compared with wild-type sperm
      • at 8 to 10 months of age, little to no sperm motility is observed compared with wild-type sperm
    • decreased male germ cell number (MGI Ref ID J:171266)
      • at 8 to 10 months of age
      • azoospermia (MGI Ref ID J:171266)
        • at 8 to 10 months of age, no mature spermatozoa are present in the epididymal epithelium unlike in wild-type mice
    • male infertility (MGI Ref ID J:171266)
    • seminiferous tubule degeneration (MGI Ref ID J:171266)
      • with vacuolation and rare giant cells
    • teratozoospermia (MGI Ref ID J:171266)
      • most sperm exhibits head defects and head-to-tail fusions unlike in wild-type mice
      • some sperm exhibit a hook-like appearance
      • older mice exhibit more severe sperm abnormalities compared with younger mice
      • abnormal acrosome morphology (MGI Ref ID J:171266)
        • incomplete in some sperm
      • abnormal sperm midpiece morp hology (MGI Ref ID J:171266)
        • a small piece is missing from the midpiece unlike in wild-type sperm
• endocrine/exocrine gland phenotype
  • seminiferous tubule degeneration (MGI Ref ID J:171266)
    • with vacuolation and rare giant cells

Founder genetic background: FVB/NTac

Strain genetic background: FVB/NTac

Strain Development:
Founder males were mated to FVB/N females. Founder line 1309-10 had high level expression and was used to produce the R26-EGFP line. Hemizygous transgenic-positive males are mated to FVB/N females to maintain the colony.

Donor: Eric Sandgren, Ph.D., University of Wisconsin

Primary Reference:

Kisseberth WC, Brettingen NT, Lohse JK, Sandgren EP. Ubiquitous expression of marker transgenes in mice and rats. Dev Biol. 1999 Oct 1;214(1):128-38. (Medline PMID: 10491262)

Coat color: White

Other:

MMRRC Breeding System: Inbred x Hemizygote

Breeding Scheme(s):

• FVB/NTac female x Hemizygous male

Generation: Not applicable

Overall Breeding Performance: Good

Viability and Fertility	Female	Male
Homozygotes are viable:	Yes	Yes
Homozygotes are fertile:	Yes	No
Heterozygotes are fertile:	Yes	Yes
Age Reproductive Decline:	Unknown	Unknown

None

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

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MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
000366-MU-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit $4,109.00 For-Profit	Litter	Recovered litter1; additional fees for any special requests.
000366-MU-SPERM Cryo-preserved spermatozoa	$437.00 Non-Profit $817.00 For-Profit	Aliquot	Approximate quantity.2
000366-MU-EMBRYO Cryo-preserved embryos	$1,038.00 Non-Profit $2,621.00 For-Profit	Aliquot	Approximate quantity3: 20-40 embryos / aliquot

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.