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Promoter: Gt(ROSA)26Sor Name: gene trap ROSA 26, Philippe Soriano Alteration at locus: Transgenic |
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Reporter: EGFP (Jelly Fish) Name: Enhanced Green Fluorescent Protein Alteration at locus: Transgenic |
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Transgene: Tg(Gt(ROSA)26Sor-EGFP)130910Eps Name: transgene insertion 130910, Eric Sandgren Alteration at locus: Transgenic |
Genetic Alterations:
Mutant mice contain the EGFP transgene under the control of the
mouse ROSA 26 promoter. The SalI-BamHI fragment of the mouse
ROSA 26 gene was inserted into the SalI/BamHI site of pEGFP-N1
(Clonetech) containing the enhanced green fluorescent protein
gene. The SalI-AflI fragment was then excised and microinjected
into FVB/N embryos to produce transgenic mice.
Genotype Determination:
ES Cell Line: Not applicable
Phenotype:
Hemizygous mice exhibit ubiquitous expression of GFP in embryos and adult mice.
Strain Type: Inbred Transgenic Mutant
Founder genetic background: FVB/NTac
Strain genetic background: FVB/NTac
Strain Development:
Founder males were mated to FVB/N females. Founder line
1309-10 had high level expression and was used to produce the
R26-EGFP line. Hemizygous transgenic-positive males are mated
to FVB/N females to maintain the colony.
Donor: Eric Sandgren, Ph.D., University of Wisconsin
Primary Reference:
Kisseberth WC, Brettingen NT, Lohse JK, Sandgren EP. Ubiquitous expression of marker transgenes in mice and rats. Dev Biol. 1999 Oct 1;214(1):128-38. (Medline PMID: 10491262)
Coat color: White
Other:
MMRRC Breeding System: Inbred x Hemizygote
Breeding Scheme(s):
Generation: Not applicable
Overall Breeding Performance: Good
| Viability and Fertility | Female | Male |
|---|---|---|
| Homozygotes are viable: | Yes | Yes |
| Homozygotes are fertile: | Yes | No |
| Heterozygotes are fertile: | Yes | Yes |
| Age Reproductive Decline: | Unknown | Unknown |
Average litter size: 8
Recommended wean age: 3 weeks
None
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 000366-MU-RESUS | Resuscitation from cryopreserved material | $1,963.00 | litter a |
| 000366-MU-SPERM | Cryopreserved spermatozoa | $424.00 | aliquot b |
| 000366-MU-EMBRYO | Cryopreserved embryo | $1,008.00 | aliquot c |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to information.
MMRRC Contact Information
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The MMRRC is a collaborative effort, funded by grants from the National Center for Research Resources,
NIH, DHHS.
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Last Modified: January 11, 2010