|
Allele Symbol: St8sia1tm1Rlp Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1/ targeted mutation 1, Richard L Proia Chromosome: 6 Alteration at locus: Targeted Mutation |
Genetic Alterations:
Lacz-neo cassette inserted into last exon of target gene.
Genotype Determination:
ES Cell Line: TC-1 (from Chuxia Deng)
Phenotype:
When combined with GalNacT knockout causes lethal sound-induced seizures.
Strain Type: Mixed Inbred Knockout
Founder genetic background: Mixed Inbred (129S6/SvEvTac x C57BL/6)F1
Strain genetic background:
Strain Development:
Donor: Dr. Richard Proia, NIDDK/NIH
Primary Reference:
Kawai H, Allende ML, Wada R, Kono M, Sango K, Deng C, Miyakawa T, Crawley JN, Werth N, Bierfreund U, Sandhoff K, Proia RL. Mice expressing only monosialoganglioside GM3 exhibit lethal audiogenic seizures. J Biol Chem. 2001 Mar 9; 276(10):6885-8. (Medline PMID: 11133999)
Coat color: Unknown
Other:
MMRRC Breeding System: Intrastrain
Breeding Scheme(s):
Generation: Unknown
Overall Breeding Performance:
| Viability and Fertility | Female | Male |
|---|---|---|
| Homozygotes are viable: | Yes | Yes |
| Homozygotes are fertile: | Yes | Yes |
| Heterozygotes are fertile: | Yes | Yes |
| Age Reproductive Decline: | Unknown | Unknown |
Average litter size: Unknown
Recommended wean age: Unknown
None
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
|
|||
| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 000037-MU-RESUS | Resuscitation from cryopreserved material | $1,906.00 | litter a |
| 000037-MU-SPERM | Cryopreserved spermatozoa | $412.00 | aliquot b |
| 000037-MU-EMBRYO | Cryopreserved embryo | $979.00 | aliquot c |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). For additional information about this strain, please email Customer Service at service@mmrrc.org or call (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls).
MMRRC Contact Information
Home Page
The MMRRC is a collaborative effort, funded by grants from the National Center for Research Resources,
NIH, DHHS.
Web forms on this site assume JavaScript enabled for Netscape 6.x, Microsoft Internet Explorer 5.5 and later versions; forms will not function as expected with earlier versions or when JavaScript is turned off.
Last Modified: October 08, 2008