Strain Detail Sheet

Phenotype:
When combined with GalNacT knockout causes lethal sound-induced seizures.

Mammalian Phenotype Terms:
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.

St8sia1^tm1Rlp/St8sia1^tm1Rlp

        involves: 129S6/SvEvTac

• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:67955)
• mice are viable and fertile, have normal growth, and exhibit no gross behavior abnormalities
• nervous system phenotype
• nervous system phenotype (MGI Ref ID J:67955)
• mice exhibit normal myelination

Mammalian Phenotype Terms:
      assigned by genotype

Nrcam^tm1Gmt/Nrcam^tm1Gmt

        either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Swiss Webster)

• nervous system phenotype
• abnormal neurite morphology (MGI Ref ID J:71828)
• isolated cerebellar cells fail to extend neurites in response to contactin, however neurites still extend in response to RPTPbeta
• small cerebellum (MGI Ref ID J:71828)
• reduced size of lobes IV and V; these lobes contained 11% less gray matter than control littermates; lobes I, II and X are also slightly reduced in size

Nrcam^tm1Gmt/Nrcam^tm1Gmt

        involves: 129S6/SvEvTac

• nervous system phenotype
• nervous system phenotype (MGI Ref ID J:136656)
• Purkinje dendrites appear normal

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.

Nrcam^tm1Gmt/Nrcam^tm1Gmt

        involves: 129S6/SvEvTac * Swiss Webster

• behavior/neurological phenotype
• abnormal learning/ memory (MGI Ref ID J:153165)
• males and females display a longer latency to reach the escape platform in the reversal phase
• abnormal motor learning (MGI Ref ID J:153165)
• in a rotarod test females, but not males, show a shorter latency to fall in retests suggesting an impairment in the improvement normally seen across trials
• abnormal spatial learning (MGI Ref ID J:153165)
• females but not males display a longer latency to find a hidden platform
• abnormal locomotor activation (MGI Ref ID J:153165)
• in a 1 h open field test females display decreased activity during the beginning of the test and increased activity towards the end of the test relative to control females
• decreased vertical activity (MGI Ref ID J:153165)
• decrease in rearing frequency in an open field in females
• abnormal social investigation (MGI Ref ID J:153165)
• males fail to show a preference for a stranger mouse over an empty cage
• nervous system phenotype
• decreased prepulse inhibition (MGI Ref ID J:153165)
• small but significant decrease in males

Strain Type: Mixed Inbred Knockout

Founder genetic background: Mixed Inbred (129S6/SvEvTac x C57BL/6)F1

Strain genetic background:

Strain Development:

• Ganglioside function
• Epilepsy research

Donor: Dr. Richard Proia, NIDDK/NIH

Primary Reference:

Kawai H, Allende ML, Wada R, Kono M, Sango K, Deng C, Miyakawa T, Crawley JN, Werth N, Bierfreund U, Sandhoff K, Proia RL. Mice expressing only monosialoganglioside GM3 exhibit lethal audiogenic seizures. J Biol Chem. 2001 Mar 9; 276(10):6885-8. (Medline PMID: 11133999)

Coat color: Unknown

Other:

MMRRC Breeding System: Intrastrain

Breeding Scheme(s):

• Homozygous Female x Homozygous Male

Generation: Unknown

Overall Breeding Performance:

Viability and Fertility	Female	Male
Homozygotes are viable:	Yes	Yes
Homozygotes are fertile:	Yes	Yes
Heterozygotes are fertile:	Yes	Yes
Age Reproductive Decline:	Unknown	Unknown

None

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
000037-MU-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit	Litter	Recovered litter1; additional fees for any special requests.
000037-MU-SPERM Cryo-preserved spermatozoa	$437.00 Non-Profit	Aliquot	Approximate quantity.2
000037-MU-EMBRYO Cryo-preserved embryos	$1,038.00 Non-Profit	Aliquot	Approximate quantity3: 20-40 embryos / aliquot

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.