Strain Detail Sheet

Phenotype:
Homozygous phenotype: The mutant mice are viable and appear without major abnormalities but show a heightened sensitivity to insulin. A basis for the increased insulin sensitivity in the mutant mice was found to be enhanced insulin receptor phosphorylation in skeletal muscle. The mutant mice were protected from high-fat diet-induced insulin resistance.

The Heterozygous phenotype is normal.

Strain Type: Non-Inbred

Founder genetic background: 129/svev X C57BL/6

Strain genetic background: 129/svev X C57BL/6

Strain Development:

• Apoptosis
• Cancer
• Dermatology
• Diabetes/Obesity
• Immunology and Inflammation
• Models for Human Disease
• Research Tools

Donor: Richard L. Proia, Ph.D., NIH, NIDDK

Primary Reference:

Yamashita T, Hashiramoto A, Haluzik M, Mizukami H, Beck S, Norton A, Kono M, Tsuji S, Daniotti JL, Werth N, Sandhoff R, Sandhoff K, Proia RL. Enhanced insulin sensitivity in mice lacking ganglioside GM3. Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3445-9. Epub 2003 Mar 10. (Medline PMID: 12629211 )

Coat color: Black and Agouti

Other:

MMRRC Breeding System: Sib mating or intra strain mating

Breeding Scheme(s):

• Homozygous female x Homozygous male

Generation: Unknown

Overall Breeding Performance: Normal

None

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
000374-MU-RESUS	Resuscitation from cryopreserved material	$1,906.00	litter a
000374-MU-SPERM	Cryopreserved spermatozoa	$412.00	aliquot b
000374-MU-EMBRYO	Cryopreserved embryo	$979.00	aliquot c

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

^c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.