Strain Detail Sheet

Phenotype:
Unknown

Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

Sall4^Gt(XE027)Byg/Sall4⁺

        B6;129P2-Sall4<Gt(XE027)Byg>

• mortality/aging
  • partial embryonic lethality during organogenesis (MGI Ref ID J:105332)
    • about 20% of heterozygotes develop a severe phenotype and die by E11.5; the rest develop a milder phenotype and are born alive
    • defects are less severe than when crossed into a Black Swiss background
• limbs/digits/tail phenotype
  • abnormal limbs/digits/tail morphology (MGI Ref ID J:105332)
    • defects are less severe than when crossed into a Black Swiss background
    • abnormal carpal bone morphology (MGI Ref ID J:105332)
      • on the forelimb multiple carpal bone defects are seen including reduced size of the trapezoid and capitate, fusion of the trapezoid with the adjacent digit 1 metacarpal, and abnormal junction of the digit 2 metacarpal with both the trapezoid and trapezium
    • abnormal digit morphology (MGI Ref ID J:105332)
      • defects are more severe on the hindlimb and include triphalangeal digit 1 or shortened digit 1
      • abnormal phalanx morphology (MGI Ref ID J:105332)
        • forelimb digit 1 proximal phalange is mildly elongated
    • abnormal limb bud morphology (MGI Ref ID J:105332)
      • at E9.5, in the more severe cases defects in hindlimb bud outgrowth are seen
    • elongated metacarpal bones (MGI Ref ID J:105332)
      • forelimb digit 1 and digit 4 metacarpals are mildly elongated
• skeleton phenotype
  • abnormal carpal bone morphology (MGI Ref ID J:105332)
    • on the forelimb multiple carpal bone defects are seen including reduced size of the trapezoid and capitate, fusion of the trapezoid with the adjacent digit 1 metacarpal, and abnormal junction of the digit 2 metacarpal with both the trapezoid and trapezium
  • abnormal phalanx morphology (MGI Ref ID J:105332)
    • forelimb digit 1 proximal phalange is mildly elongated
  • elongated metacarpal bones (MGI Ref ID J:105332)
    • forelimb digit 1 and digit 4 metacarpals are mildly elongated
• embryogenesis phenotype
  • abnormal limb bud morphology (MGI Ref ID J:105332)
    • at E9.5, in the more severe cases defects in hindlimb bud outgrowth are seen

Sall4^Gt(XE027)Byg/Sall4^Gt(XE027)Byg

        B6;129P2-Sall4<Gt(XE027)Byg>

• mortality/aging
  • complete embryonic lethality before somite formation (MGI Ref ID J:138922)
    • arrest between E5.75 and E6.0
  • complete embryonic lethality before somite formation (MGI Ref ID J:105332)
    • die before E8.0
• embryogenesis phenotype
  • embryonic growth arrest (MGI Ref ID J:138922)
    • arrest between E5.75 and E6.0

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.

Sall4^Gt(XE027)Byg/Sall4⁺

        involves: 129P2/OlaHsd * Black Swiss * C57BL/6

• mortality/aging
  • embryonic lethality during organogenesis (MGI Ref ID J:105332)
    • the severity of defects is increased when crossed into a Black Swiss background
• limbs/digits/tail phenotype
  • abnormal limbs/digits/tail morphology (MGI Ref ID J:105332)
    • the severity of defects is increased when crossed into a Black Swiss background
• cardiovascular system phenotype
  • abnormal heart development (MGI Ref ID J:105332)
    • at E9.5 in severely affected embryos defects in cardiac patterning can be seen including decreased expression of Gja5 and expansion of the left ventricular marker Nppa across the interventricular groove
  • abnormal interventricular groove (MGI Ref ID J:105332)
    • the interventricular groove is less pronounced or in severe cases absent
  • common ventricle (MGI Ref ID J:105332)
    • in severe cases a univentricular heart is present with thin myocardium
  • disorganized myocardium (MGI Ref ID J:105332)
    • in less severe cases the myocardium is disorganized
  • muscular ventricular septal defect (MGI Ref ID J:105332)
    • heterozygotes that survive past E11.5 often have ventricular septal defects
  • thin myocardium (MGI Ref ID J:105332)
    • in severe cases a univentricular heart is present with thin myocardium

Founder genetic background: C57BL/6J; 129P2/OlaHsd

Strain genetic background: C57BL/6J; 129P2/OlaHsd

Strain Development:
This sequence was obtained by 5' RACE PCR from a 129P2/OlaHsd mouse embryonic stem cell line with an insertional mutation from gene trap vector pGT1Lxf . The ES cell line was injected into C57BL/6J host blastocysts to generate chimeras. The chimeras were then crossed with C57BL/6J mice to determine germline transmission.

Donor: MMRRC at UC Davis

Primary Reference:

Coat color: Agouti/Black
(Note: if sibling mated, it is possible to get pink-eyed chinchilla, the color of the 129P2/Ola, or other mixed variations.)

Other:

MMRRC Breeding System: Sib mating or Backcross

Breeding Scheme(s):

• Heterozygous female x Wildtype male
• Wildtype female x Heterozygous male
• Heterozygous female x Wildtype male
• Wildtype female x Heterozygous male

Generation: N1

Overall Breeding Performance: Not provided

Viability and Fertility	Female	Male
Homozygotes are viable:	n/a*	n/a*
Homozygotes are fertile:	n/a*	n/a*
Heterozygotes are fertile:	n/a*	Yes
Age Reproductive Decline:	n/a*	n/a*

* Matings being done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the background strain

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

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MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
000405-UCD-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit	Litter	Recovered litter1; additional fees for any special requests.
000405-UCD-SPERM Cryo-preserved spermatozoa	$437.00 Non-Profit	Aliquot	Approximate quantity.2

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.