Strain Detail Sheet

Phenotype:
Unknown

Strain Type: Mixed Inbred Induced Mutant

Founder genetic background: C57BL/6J; 129P2/OlaHsd

Strain genetic background: C57BL/6J; 129P2/OlaHsd

Strain Development:
This sequence was obtained by 5' RACE PCR from a 129P2/OlaHsd mouse embryonic stem cell line with an insertional mutation from gene trap vector pGT1Lxf . The ES cell line was injected into C57BL/6J host blastocysts to generate chimeras. The chimeras were then crossed with C57BL/6J mice to determine germline transmission.

• Developmental biology
• Radial limb defects
• Okihiro syndrome
• Duane anomaly
• Hearing loss

Donor: MMRRC at UC Davis

Primary Reference:

Coat color: Agouti/Black
(Note: if sibling mated, it is possible to get pink-eyed chinchilla, the color of the 129P2/Ola, or other mixed variations.)

Other:

MMRRC Breeding System: Sib mating or Backcross

Breeding Scheme(s):

• Heterozygous female x Wildtype male
• Wildtype female x Heterozygous male
• Heterozygous female x Wildtype male
• Wildtype female x Heterozygous male

Generation: N1

Overall Breeding Performance: Not provided

* Matings being done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the background strain

* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item #	Description	Distribution Fee/unit (US $)	Units
000405-UCD-RESUS	Resuscitation from cryopreserved material	$1,906.00	litter a
000405-UCD-SPERM	Cryopreserved spermatozoa	$412.00	aliquot b
000405-UCD-EMBRYO	Cryopreserved embryo	$979.00	aliquot c

^a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.

^b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

^c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.