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Allele Symbol: Sgcdtm1Kcam Name: sarcoglycan, delta (dystrophin-associated glycoprotein) / targeted mutation 1, Kevin P Campbell Alteration at locus: Knockout |
Genetic Alterations:
A targeting vector
containing a neo cassette was inserted to replace exon 2, which
encodes for 63 amino acids of the intracelular domain and the
entire transmembrane region.
Genotype Determination:
ES Cell Line: R1
Phenotype:
Homozygous Phenotype: Sgcd null mice show a phenotype consistent with human limb
girdle muscular dystrophy. Pathology includes large regions of
necrosis in nearly all skeletal muscles. Severe necrotic lesions
are present in diaphragm of mice under 1 month of age. Mice older
than 6 months of age display cardiomyopathy. Mice display
irregularities of the coronary vasculature.
Heterozygous Phenotype: Heterozygotes do not show any known morphological abnormalities.
Strain Type:
Founder genetic background: 129 x C57BL/6 hybrid
Strain genetic background: C57BL/6J
Strain Development:
Donor: Kevin P. Campbell, Ph.D., University of Iowa
Primary Reference:
Coral-Vazquez R, Cohn RD, Moore SA, Hill JA, Weiss RM, Davisson RL, Straub V, Barresi R, Bansal D, Hrstka RF, Williamson R, Campbell KP. Disruption of the sarcoglycan-sarcospan complex in vascular smooth muscle: a novel mechanism for cardiomyopathy and muscular dystrophy. Cell. 1999 Aug 20;98(4):465-74. (Medline PMID: 10481911)
Cohn RD, Durbeej M, Moore SA, Coral-Vazquez R, Prouty S, Campbell KP. Prevention of cardiomyopathy in mouse models lacking the smooth muscle sarcoglycan-sarcospan complex. J Clin Invest. 2001 Jan;107(2):R1-7. (Medline PMID: 11160141)
Mice with decreased mobility may benefit from gruel as food/water source.
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain.
(Use the MMRRC
Catalog Search to request more than one strain.)
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 000406-MU-RESUS | Resuscitation from cryopreserved material | $1,963.00 | litter a |
| 000406-MU-SPERM | Cryopreserved spermatozoa | $424.00 | aliquot b |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). For additional information about this strain, please email Customer Service at service@mmrrc.org or call (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls).
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Last Modified: June 19, 2009