Strain Detail Sheet

Phenotype:
Homozygous phenotype: alpha1 3.2-null mice have constitutively constricted coronary arterioles and focal myocardial fibrosis. Coronary arteries isolated from alpha1 3.2-null arteries showed normal contractile responses but reduced relaxation in response to acetylcholine and nitroprusside.

Heterozygous phenotype: Heterozygotes do not show any morphological abnormalities.

Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.

Cacna1h^tm1Kcam/Cacna1h^tm1Kcam

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• cardiovascular system phenotype
  • abnormal cardiovascular system morphology (MGI Ref ID J:86616)
    • abnormal blood vessel morphology (MGI Ref ID J:86616)
      • vessels in the heart have irregular spiral shapes
      • abnormal arteriole morphology (MGI Ref ID J:86616)
        • exhibit constitutively constricted coronary arterioles
    • abnormal myocardial fiber morphology (MGI Ref ID J:86616)
      • focal myocardial fibrosis
    • cardiac fibrosis (MGI Ref ID J:86616)
      • exhibit progressive cardiac fibrosis starting at 10 weeks of age; hearts show large areas of fibrosis, necrosis, and lymphoctye infiltration
  • increased vasoconstriction (MGI Ref ID J:86616)
    • vessels in the heart and coronary arterioles are constitutively contracted
    • isolated coronary arteries show normal contractile responses but show reduced relaxation in response to acetylcholine and ntiroprusside
• growth/size phenotype
  • decreased body size (MGI Ref ID J:86616)
    • both males and females are smaller
• nervous system pheno type
  • abnormal channel response (MGI Ref ID J:86616)
    • the low voltage-activated calcium current (I_T is significantly diminished in dorsal root ganglia neurons, however no difference in the high voltage-activated calcium currents
• muscle phenotype
  • abnormal myocardial fiber morphology (MGI Ref ID J:86616)
    • focal myocardial fibrosis
  • increased vasoconstriction (MGI Ref ID J:86616)
    • vessels in the heart and coronary arterioles are constitutively contracted
    • isolated coronary arteries show normal contractile responses but show reduced relaxation in response to acetylcholine and ntiroprusside

Founder genetic background: C57BL/6x129

Strain genetic background: C57BL/6

Strain Development:

Donor: Kevin P. Campbell, Ph.D., University of Iowa

Primary Reference:

Chen CC, Lamping KG, Nuno DW, Barresi R, Prouty SJ, Lavoie JL, Cribbs LL, England SK, Sigmund CD, Weiss RM, Williamson RA, Hill JA, Campbell KP. Abnormal coronary function in mice deficient in alpha1H T-type Ca2+ channels. Science. 2003 Nov 21;302(5649):1416-8. (Medline PMID: 14631046)

The use of mouse houses help to facilitate better reproductive results. Once the pups are weaned at 21 days, they should be grueled for about one week.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.) Request this Strain
MMRRC Item # Description	Distribution Fee/unit (US $)	Units	Notes
009979-MU-RESUS Litter recovered from cryo-archive	$2,022.00 Non-Profit	Litter	Recovered litter1; additional fees for any special requests.
009979-MU-SPERM Cryo-preserved spermatozoa	$437.00 Non-Profit	Aliquot	Approximate quantity.2

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.