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Allele Symbol: Cacna1htm1Kcam Name: calcium channel, voltage-dependent, T type, alpha 1H subunit / targeted mutation 1, Kevin P Campbell Alteration at locus: Knockout |
Genetic Alterations:
The long arm of homology in the targeting vector was a 4.5 kb NotI/EcoRI fragment
upstream of the IS4 region of alpha1 3.2 subunit, which has been subcloned into
pBluescript KS (+) (pBS) and cut with NotI/ClaI. The short arm was a 2.2 kb
BamHI/BamHI fragment downstream of the extracellular loop between IS5 and IS6.
These fragments were inserted into cloning sites of pPNT flanking a reversed
PGK-neomycin resistance cassette. The vector included a thymidine kinase
cassette distal to the short arm to allow for negative selection of
non-recombinants.
Genotype Determination:
ES Cell Line: R1
Phenotype:
Homozygous phenotype: alpha1 3.2-null mice have constitutively constricted coronary arterioles and focal
myocardial fibrosis. Coronary arteries isolated from alpha1 3.2-null arteries showed
normal contractile responses but reduced relaxation in response to acetylcholine
and nitroprusside.
Heterozygous phenotype: Heterozygotes do not show any morphological abnormalities.
Strain Type:
Founder genetic background: C57BL/6x129
Strain genetic background: C57BL/6
Strain Development:
Donor: Kevin P. Campbell, Ph.D., University of Iowa
Primary Reference:
Chen CC, Lamping KG, Nuno DW, Barresi R, Prouty SJ, Lavoie JL, Cribbs LL, England SK, Sigmund CD, Weiss RM, Williamson RA, Hill JA, Campbell KP. Abnormal coronary function in mice deficient in alpha1H T-type Ca2+ channels. Science. 2003 Nov 21;302(5649):1416-8. (Medline PMID: 14631046)
The use of mouse houses help to facilitate better reproductive results. Once the pups are weaned at 21 days, they should be grueled for about one week.
Mice recovered from a cryo-archive will have health surveillance performed on the
resuscitated animals.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
* Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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| MMRRC Item # | Description | Distribution Fee/unit (US $) |
Units |
|---|---|---|---|
| 009979-MU/H-RESUS | Resuscitation from cryopreserved material | $1,906.00 | litter a |
| 009979-MU/H-SPERM | Cryopreserved spermatozoa | $412.00 | aliquot b |
| 009979-MU/H-EMBRYO | Cryopreserved embryo | $979.00 | aliquot c |
a The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one breedable mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC repository will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC repository.
b An aliquot contains sufficient material (volume and concentration) for at least two IVF or several artificial inseminations (based on our procedures). The MMRRC makes no guarantee concerning success of these procedures when performed outside the MMRRC facilities.
c An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). For additional information about this strain, please email Customer Service at service@mmrrc.org or call (800) 910-2291 (in USA or Canada) or (207) 288-6009 (international calls).
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Last Modified: July 17, 2008