Strain Detail Sheet

Strain Name:

Sanger MirKO ES cell line Mir23b/Mir27b cluster 13N2

Stock Number:

036364-UCD

Other Names:

13N2

Gene Information

Gene Detail

**This cell line is a cluster knockout in which more than one gene has been targeted**
Gene Symbol: Mir23b
Name: microRNA 23b
Alteration at locus: Knockout
Chromosome: 13
Gene Symbol: Mir27b
Name: microRNA 27b
Alteration at locus: Knockout
Chromosome: 13

Genetic Alterations:
The cluster of microRNA genes was replaced via homologous recombination by a LoxP-F3-PGK-EM7-PuroΔtk-bpA-LoxP-FRT targeting vector with 5.5kb (5’) and 3.3kb (3’) arms of homology. Briefly, this is a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters and flanked by LoxP and F3/FRT sites. Schematic of MirKO targeting vector and alleles


Mouse strain from which the parental cell line was derived:

Related MMRRC Products:
Mir23b: MMRRC:036363-UCD Sanger MirKO ES cell line Mir23b-Mir24-1 cluster 13N5
Mir23b: MMRRC:036865-UCD Keck MirKO ES cell line Mirc22
Please see also: Sanger MicroRNA Knockout ES Cell Lines Overview

Genotype Determination:

Related Protocols

Strain Origin

Donor: Bradley, Ph.D., Allan, Wellcome Trust Sanger Institute, UK

Primary Reference:

Prosser HM, Koike-Yusa H, Cooper JD, Law FC, Bradley A. A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nature Biotechnology 2011, 29(9):840-5. (Medline PMID:21822254).

Special Considerations

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

- Products for this strain are Not Yet Available for Ordering -
If you register interest in this strain, you will be notified when it becomes available for ordering.
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
036364-UCD-CELLSanger MirKO ES cell line Mir23b/Mir27b cluster 13N2
$546.00
Non-Profit
Vial Without genetic confirmation.*
recommended serviceMutant ES cell line genetic confirmation service
$279.00
Non-Profit
per cell line Genetic confirmation recommended.*Service fee is in addition to fee for mutant cell line.
available serviceBasic microinjection service
$3,317.00
Non-Profit
per cell line In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed.

*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation of the reporter sequence.

This strain is not currently available. If you register interest in this strain, you will be notified when it becomes available for ordering (click Register Interest button above).



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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