Strain Detail Sheet

Strain Name:

SIGTR ES cell line XP0209

Stock Number:

025459-UCD

Other Names:

XP0209

Gene Information

Gene Detail

Gene Symbol: Sumo3
Name: SMT3 suppressor of mif two 3 homolog 3 (yeast)
Alteration at locus: Gene Trap
Chromosome: 10

Genetic Alterations:
High throughput gene trapping was performed by inserting a gene trap vector containing a splice-acceptor sequence upstream of a reporter gene, β-geo (a fusion of β-galactosidase and neomycin phosphotransferase II), into an intronic or coding region of genomic DNA. The resulting insertional mutation creates a fusion transcript containing sequences from exons upstream of the insertion joined to the β-geo marker, allowing cell lines where the vector has successfully interrupted a gene to be identified.

Parental ES Cell Line: E14Tg2a.4 (MMRRC Stock Number 015890-UCD)
Mouse strain from which the parental cell line was derived:

Related MMRRC Products:
Sumo3: MMRRC:006713-UCD BayGenomics ES cell line XB039
Sumo3: MMRRC:006758-UCD BayGenomics ES cell line XB216
Sumo3: MMRRC:017883-UCD SIGTR ES cell line AW0265
Sumo3: MMRRC:034248-UCD B6;129P2-Sumo3Gt(XP0209)Wtsi/Mmucd
Please also see: MMRRC Overview of the Sanger Institute Gene Trap Resource ES Cell Line collection

Check to access IGTC Cell Line Annotation

Related Protocols

Strain Origin

Donor: Wellcome Trust Sanger Institute, The Sanger Institute Gene Trap Resource (SIGTR), Cambridge, UK

Primary Reference:

Skarnes WC, von Melchner H, Wurst W, Hicks G, Nord AS, Cox T, Young SG, Ruiz P, Soriano P, Tessier-Lavigne M, Conklin BR, Stanford WL, Rossant J, International Gene Trap Consortium. A public gene trap resource for mouse functional genomics. Nat Genet. 2004 Jun;36(6):543-4. (Medline PMID: 15167922)

Special Considerations

When choosing a clone, we recommend blasting the sequence tag to estimate the location of a gene trap. Generally you will want to select a clone that traps the furthest upstream. The vast majority of gene trap cell lines have been correctly identified, as judged by DNA sequencing (i.e., repeat determination of the DNA sequence tag when the ES cell clone is requested). However, incorrectly identified ES cell clones are occasionally encountered, either because of human error or because a thawed ES cell line gets overgrown by a contaminating ES cell line. Therefore, we strongly recommend that you request verification of the identity of your ES cell line by DNA sequencing (there is an additional fee for DNA sequence verification of your clone). We also recommend that recipients of ES cells re-verify the identity of the ES cell line by RT-PCR and/or Southern blot analysis. If more than one ES cell line is available for your "gene-of-interest", we recommend that you order several of the ES cell lines. Ordering more than one ES cell line for a specific "gene-of-interest" will increase the chances of success.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. The parental cell line E14Tg2a.4, from which these cells were derived, was tested for multiple mouse pathogens; please see the Health Status Report. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.

Conditions of Distribution

Please complete the Sanger Institute Gene Trap MTA. This document must be signed by an authorized Technology Transfer representative from your institution. Please provide two signed originals, mailed to the address located on page 2 of the MTA. The MTA will be counter-signed by a designated representative from the MMRRC, and a fully executed copy returned to your institution. Your request will be processed upon receipt of the signed MTA at the appropriate MMRRC center.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
025459-UCD-CELLSIGTR ES cell line XP0209
$546.00
Non-Profit
Vial Without genetic confirmation.*
recommended serviceMutant ES cell line genetic confirmation service
$279.00
Non-Profit
per cell line Genetic confirmation recommended.*Service fee is in addition to fee for mutant cell line.
available serviceBasic microinjection service
$3,317.00
Non-Profit
per cell line In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed.
available serviceGuaranteed microinjection service
$5,540.00
Non-Profit
per cell line In addition to the per mutant ES cell line fee (see above); at least one 50% or greater chimeric male, or not charged at all.
015890-UCD-CELLLow Passage wild type parental clone E14Tg2a.4 suitable for a single electroporation and germline transmission
$546.00
Non-Profit
Vial
015890-UCD-CTRLHigh Passage wild type parental clone E14Tg2a.4 suitable for DNA extraction, control purposes, but not for germline transmission
$56.00
Non-Profit
Vial
015890-UCD-ULTRAUltra Low Passage wild type parental clone E14Tg2a.4 suitable for multiple electroporations and germline transmission
$1,592.00
Non-Profit
Vial

*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation of the reporter sequence.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-7510 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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