Strain Name:
C57BL/6-Rc3h1san/Mmucd
Stock Number:
011119-UCD
Citation ID:
RRID:MMRRC_011119-UCD
Other Names:
San Roque, C57BL/6-Rc3h1san/Mmcd, C57BL/6JSfdAnu-Rc3h1san/AnuApb

Strain Information

Rc3h1san
Name: RING CCCH (C3H) domains 1; sanroque
Synonyms: San Roque, Roquinsan
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
Rc3h1
Name: RING CCCH (C3H) domains 1
Synonyms: roquin, 5730557L09Rik
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 381305
Homologene: 19036
Genetic Alterations
The San Roque mutation corresponds to a T to G transversion at position 981 in exon 5 of 20 total exons. This mutation results in a Methionine to Arginine amino acid change at position 199 of the RC3H1 protein.
Genotype Determination
Phenotype
Homozygous: Spontaneous autoimmune disease. Systemic lupus erythematosus. Anti-nuclear autoantibodies (ANA), hypergammaglobulinemia, autoimmune nephritis, lymphadenopathy, increased numbers of CD44high activated memory T cells, increased numbers of T follicular helper cells, spontaneous germinal centres, CD28-independent activation of T cells. Increased ICOS mRNA and protein expression on T cells, defect in micro-RNA (mir) inhibition of mRNA stability.

Heterozygous: Low penetrance in hets
Mammalian Phenotype Terms
Allelic Composition: Rc3h1san/Rc3h1san (Genetic Background: either: C57BL/6JSfdAnu-Rc3h1san/Anu or (involves: C57BL/6JSfdAnu * CBA/Ca) )

Allelic Composition: Rc3h1san/Rc3h1san (Genetic Background: involves: C57BL/6JSfdAnu )

Allelic Composition: Rc3h1san/Rc3h1+ (Genetic Background: involves: C57BL/6JSfdAnu )

Allelic Composition: Rc3h1tm1.2Cgv/Rc3h1san (Genetic Background: involves: C57BL/6 * C57BL/6JSfdAnu )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Autoantibodies/immunology
  • Autoimmunity/immunology
  • Cell Line, Tumor
  • Disease Models, Animal
  • Female
  • Humans
  • Lupus Erythematosus, Systemic/genetics
  • Lupus Erythematosus, Systemic/immunology
  • Lupus Erythematosus, Systemic/metabolism
  • Lupus Erythematosus, Systemic/pathology
  • Male
  • Mice
  • Molecular Sequence Data
  • Mutation, Missense/genetics
  • Receptors, Antigen, T-Cell/immunology
  • Receptors, Antigen, T-Cell/metabolism
  • T-Lymphocytes, Helper-Inducer/immunology
  • T-Lymphocytes, Helper-Inducer/metabolism
  • T-Lymphocytes, Helper-Inducer/pathology
  • Ubiquitin-Protein Ligases/chemistry
  • Ubiquitin-Protein Ligases/classification
  • Ubiquitin-Protein Ligases/genetics
  • Ubiquitin-Protein Ligases/metabolism
  • 3' Untranslated Regions/genetics
  • 3' Untranslated Regions/metabolism
  • Antigens, Differentiation, T-Lymphocyte/genetics
  • Antigens, Differentiation, T-Lymphocyte/metabolism
  • Autoimmunity/genetics
  • Base Sequence
  • Cell Line
  • Gene Expression Regulation
  • Inducible T-Cell Co-Stimulator Protein
  • MicroRNAs/genetics
  • MicroRNAs/metabolism
  • Mutation
  • RNA, Messenger/biosynthesis
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Regulatory Sequences, Ribonucleic Acid/genetics
  • T-Lymphocytes/metabolism
  • Alleles
  • Antigens, Differentiation, T-Lymphocyte/immunology
  • CD28 Antigens/genetics
  • CD28 Antigens/immunology
  • CD28 Antigens/metabolism
  • Cell Differentiation/immunology
  • Germinal Center/immunology
  • Immunologic Memory/immunology
  • Interleukin-2/immunology
  • Interleukin-2/metabolism
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mutation/genetics
  • Salmonella/immunology
  • Signal Transduction/immunology
  • T-Lymphocytes/cytology
  • T-Lymphocytes/immunology
  • Thymus Gland/cytology
  • Thymus Gland/immunology
  • Ubiquitin-Protein Ligases/immunology
  • Autoantibodies/blood
  • B-Lymphocytes/cytology
  • B-Lymphocytes/immunology
  • Cell Count
  • Cell Proliferation
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/metabolism
  • Germinal Center/cytology
  • Heterozygote
  • Interleukins/deficiency
  • Lupus Erythematosus, Systemic/blood
  • Lymphocyte Activation
  • Lymphocyte Subsets/cytology
  • Lymphocyte Subsets/immunology
  • Phenotype
  • Proto-Oncogene Proteins c-bcl-6
  • T-Lymphocytes, Helper-Inducer/transplantation
  • Th1 Cells/cytology
  • Th1 Cells/immunology
  • Th2 Cells/cytology
  • Th2 Cells/immunology
Strain Development
Mutation induced in male C57BL/6 mouse; maintained as C57BL/6 but has been crossed with many other strains as well.
Suggested Control Mice
  • Wild-type littermates
  • C57BL/6
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell biology
  • Immunology and Inflammation
  • Models for human disease
  • Systemic lupus erythematosus (SLE)
Donor
Chris Goodnow, BVSc, PhD, The Australian National University, Canberra, Australia
Primary Reference
  • Vinuesa CG, Cook MC, Angelucci C, Athanasopoulos V, Rui L, Hill KM, Yu D, Domaschenz H, Whittle B, Lambe T, Roberts IS, Copley RR, Bell JI, Cornall RJ, Goodnow CC. A RING-type ubiquitin ligase family member required to repress follicular helper T cells and autoimmunity. Nature. 2005 May 26;435(7041):452-8. (Medline PMID: 15917799)
  • Yu D, Tan AH, Hu X, Athanasopoulos V, Simpson N, Silva DG, Hutloff A, Giles KM, Leedman PJ, Lam KP, Goodnow CC, Vinuesa CG. Roquin represses autoimmunity by limiting inducible T-cell co-stimulator messenger RNA. Nature. 2007 Nov 8;450(7167):299-303. ( Medline PMID: 18172933)
  • Linterman M, Rigby RJ, Wong RK, Silva D, Withers D, Anderson G, Brink R, Hutloff A, Goodnow CC & Vinuesa CG. Roquin differentiates the specialized functions of duplicated T cell co-stimulatory receptor genes Cd28 and ICOS. Immunity. 2009 Feb;30(2):228-41. Epub 2009 Feb 12. (Medline PMID: 19217324)
  • Linterman MA; Rigby RJ; Wong RK; Yu D; Brink R; Cannons JL; Schwartzberg PL; Cook MC; Walters GD; Vinuesa CG, Follicular helper T cells are required for systemic autoimmunity. J Exp Med. 2009 Mar 16;206(3):561-76. (Medline PMID: 19221396)

Colony and Husbandry Information

No loud music.

This strain is also reposited as San Roque at the NHMRC Australian PhenomeBank (APB).

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Other or uncertain
Overall Breeding Performance
Good
Average litter size
6-8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
011119-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
011119-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
011119-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.