Strain Name:
C57BL/6-Tg(MSR1-Cyp7a1)1Road/Mmucd
Stock Number:
012049-UCD
Citation ID:
RRID:MMRRC_012049-UCD
Other Names:
7aMac, C57BL/6-Tg(MSR1-Cyp7a1)1Road/Mmcd

Strain Information

Msr1
Name: macrophage scavenger receptor 1
Synonyms: SR-AII, SR-AI, MSR-A, Scara1, Scvr, MRS-A
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20288
HGNC: HGNC:7376
Homologene: 12822
Cyp7a1
Name: cytochrome P450, family 7, subfamily a, polypeptide 1
Type: Gene
Species: Rattus norvegicus (norway rat)
Chromosome: 5
Tg(MSR1-Cyp7a1)1Road
Name: transgene insertion 1, Roger A Davis
Synonyms: 7aMac
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
7aMac express either CYP7A1 or paraoxonase1 transgenes only in macrophages due to use of the human SRA (macrophage specific) promoter element.
Genotype Determination
Phenotype
Homozygous: undetermined

Hemizygous: High expression of CYP7A1 in macrophages (7aMac).
MeSH Terms
  • Animals
  • Apolipoprotein B-100
  • Apolipoproteins B/biosynthesis
  • Apolipoproteins B/blood
  • Apolipoproteins B/metabolism
  • Bile Acids and Salts/metabolism
  • CCAAT-Enhancer-Binding Proteins/biosynthesis
  • CCAAT-Enhancer-Binding Proteins/genetics
  • Carrier Proteins/metabolism
  • Cholesterol/metabolism
  • Cholesterol 7-alpha-Hydroxylase/genetics
  • Cholesterol 7-alpha-Hydroxylase/physiology
  • DNA-Binding Proteins/biosynthesis
  • DNA-Binding Proteins/genetics
  • Hyperlipidemias/blood
  • Lipid Metabolism
  • Lipids/blood
  • Liver/enzymology
  • Liver/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • RNA, Messenger/biosynthesis
  • Receptors, LDL/biosynthesis
  • Receptors, LDL/genetics
  • Sterol Regulatory Element Binding Protein 1
  • Sterol Regulatory Element Binding Protein 2
  • Taurochenodeoxycholic Acid/metabolism
  • Transcription Factors/biosynthesis
  • Transcription Factors/genetics
  • Triglycerides/blood
  • Arteriosclerosis/etiology
  • Arteriosclerosis/metabolism
  • Arteriosclerosis/prevention & control
  • Cholelithiasis/etiology
  • Cholelithiasis/metabolism
  • Cholelithiasis/prevention & control
  • Cholesterol/blood
  • Cholesterol 7-alpha-Hydroxylase/biosynthesis
  • Cholesterol, HDL/metabolism
  • Diet, Atherogenic
  • Disease Susceptibility
  • Enzyme Repression
  • Female
  • Gallbladder/metabolism
  • Homeostasis
  • Lipoproteins/metabolism
  • Male
  • Sex Characteristics
  • Aryldialkylphosphatase/genetics
  • Atherosclerosis/metabolism
  • Atherosclerosis/physiopathology
  • Bile Acids and Salts/pharmacology
  • Carcinoma, Hepatocellular
  • Cell Line, Tumor
  • DNA-Binding Proteins/metabolism
  • Gene Expression Regulation, Enzymologic/drug effects
  • Humans
  • Lipoproteins, HDL/blood
  • Liver/drug effects
  • Liver/physiology
  • Liver Neoplasms
  • Mice, Inbred C3H
  • RNA, Messenger/metabolism
  • Receptor, Fibroblast Growth Factor, Type 4/metabolism
  • Receptors, Cytoplasmic and Nuclear
  • Signal Transduction/drug effects
  • Signal Transduction/physiology
  • Toll-Like Receptor 4/metabolism
  • Transcription Factors/metabolism
  • ATP Binding Cassette Transporter 1
  • ATP Binding Cassette Transporter, Sub-Family G, Member 1
  • ATP-Binding Cassette Transporters/genetics
  • ATP-Binding Cassette Transporters/metabolism
  • Biological Transport
  • Cell Adhesion Molecules
  • Cell Division
  • Cell Survival
  • Cholesterol 7-alpha-Hydroxylase/metabolism
  • Enzyme Activation
  • Gene Expression
  • Hydroxymethylglutaryl CoA Reductases/metabolism
  • Lipoproteins, LDL/metabolism
  • Macrophages/metabolism
  • Receptors, LDL/metabolism
  • Receptors, Scavenger
  • Scavenger Receptors, Class A
  • Transfection
  • Arteriosclerosis/enzymology
  • Arteriosclerosis/genetics
  • Arteriosclerosis/pathology
  • Arteriosclerosis/therapy
  • Base Sequence
  • Bone Marrow Transplantation
  • DNA, Complementary/genetics
  • Gadolinium/pharmacology
  • Genetic Therapy
  • Kupffer Cells/drug effects
  • Kupffer Cells/enzymology
  • Kupffer Cells/pathology
  • Mice, Knockout
  • Receptors, LDL/deficiency
Strain Development
Mouse embryos from 129X1/Svj were injected with purified DNA containing the coding region of either rat CYP7A1 or mouse paraoxonase1. Progeny bearing the transgene (determined by PCR of genomic DNA) were breed with C57BL/6J mice.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Diabetes/Obesity
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
Donor
Roger A. Davis, Ph.D., San Diego State University
Primary Reference
  • Miyake JH, Doung XD, Strauss W, Moore GL, Castellani LW, Curtiss LK, Taylor JM, Davis RA. Increased production of apolipoprotein B-containing lipoproteins in the absence of hyperlipidemia in transgenic mice expressing cholesterol 7alpha-hydroxylase. J Biol Chem. 2001 Jun 29;276(26):23304-11. Epub 2001 Apr 25. Erratum in: J Biol Chem 2001 Nov 2;276(44):41526. (Medline PMID: 11323427)
  • Miyake JH, Duong-Polk XT, Taylor JM, Du EZ, Castellani LW, Lusis AJ, Davis RA. Transgenic expression of cholesterol-7-alpha-hydroxylase prevents atherosclerosis in C57BL/6J mice. Arterioscler Thromb Vasc Biol. 2002 Jan;22(1):121-6. (Medline PMID: 11788471)
  • Gutierrez A, Ratliff EP, Andres AM, Huang X, McKeehan WL, Davis RA. Bile acids decrease hepatic paraoxonase 1 expression and plasma high-density lipoprotein levels via FXR-mediated signaling of FGFR4. Arterioscler Thromb Vasc Biol. 2006 Feb;26(2):301-6. Epub 2005 Nov 10. (Medline PMID: 16284190)
  • Moore GL, Davis RA. Expression of cholesterol-7alpha-hydroxylase in murine macrophages prevents cholesterol loading by acetyl-LDL. J Lipid Res. 2002 Apr;43(4):629-35. (Medline PMID: 11907146)
  • Bradshaw G, Gutierrez A, Miyake JH, Davis KR, Li AC, Glass CK, Curtiss LK, Davis RA. Facilitated replacement of Kupffer cells expressing a paraoxonase-1 transgene is essential for ameliorating atherosclerosis in mice. Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):11029-34. Epub 2005 Jul 25. (Medline PMID: 16043712)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
012049-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
012049-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
012049-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.