Strain Name:
B6.Cg-Tg(CMV-PRKR)1Wcl/Mmmh
Stock Number:
000132-MU
Citation ID:
RRID:MMRRC_000132-MU
Major Collection:

Strain Information

PRKR
Name: protein kinase, interferon-induced, double-stranded RNA-activated
Type: Gene
Species: Homo sapiens (human)
Chromosome: 2
CMV
Name: cytomegalovirus
Type: Regulatory Region
Species: Virus
Chromosome:
Tg(CMV-PRKR)1Wcl
Name: transgene insertion 1, Warren C Ladiges
Synonyms: Tg(CMV-PRKR)1Wcl, Tg(CMV-EIF2AK2)1Wcl
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Human PKR cDNA under control of the CMV promoter
Genotype Determination
Phenotype
Spleen cells are resistant to apoptosis when treated with Actinomycin D, and show a decrease in proliferation in response to concanavalin A.
MeSH Terms
  • Animals
  • Apoptosis/drug effects
  • Bromodeoxyuridine/pharmacokinetics
  • Cell Division/drug effects
  • Cells, Cultured
  • Concanavalin A/pharmacology
  • Dactinomycin/pharmacology
  • Gene Expression
  • HSP40 Heat-Shock Proteins
  • Humans
  • Mice/genetics
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Mitogens/pharmacology
  • Phenotype
  • Protein Synthesis Inhibitors/pharmacology
  • Spleen/cytology
  • Tissue Distribution
  • eIF-2 Kinase/analysis
  • eIF-2 Kinase/genetics
  • eIF-2 Kinase/metabolism
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Developmental Biology
  • Metabolism
  • Neurobiology
  • Virology
  • Biology of Aging
Donor
Warren C. Ladiges, D.V.M., University of Washington.
Primary Reference
Ladiges W, Morton J, Hopkins H, Wilson R, Filley G, Ware C, Gale M. Expression of human PKR protein kinase in transgenic mice. J Interferon Cytokine Res. 2002 Mar;22(3):329-34. (Medline PMID: 12034040)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • C57BL/6 female x Hemizygous carrier male
Generation
N7
Overall Breeding Performance
Good
Average litter size
6
Recommended wean age
3 - 4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000132-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000132-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000132-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.