Strain Name:
FVB-Tg(Ckm-PTPRF)13Bbk/Mmucd
Stock Number:
015507-UCD
Citation ID:
RRID:MMRRC_015507-UCD
Other Names:
MCK-hLAR, FVB-Tg(Ckm-PTPRF)13Bbk/Mmcd

Strain Information

Ckm
Name: creatine kinase, muscle
Synonyms: Ckmm, M-CK, MCK
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12715
HGNC: HGNC:1994
Homologene: 20432
PTPRF
Name: protein tyrosine phosphatase, receptor type, F
Type: Gene
Species: Homo sapiens (human)
Chromosome: 1
Tg(Ckm-PTPRF)13Bbk
Name: transgene insertion 13, Barbara B Kahn
Synonyms: MCK-hLAR
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Mice express the cDNA of the human isoform of the leukocyte antigen-related protein tyrosine phosphatase (LAR or PTPRF) from the muscle creatine kinase promoter. Mice overexpress LAR 2-fold in skeletal muscle and at a very low level in cardiac tissue.
Genotype Determination
Phenotype
Homozygous: Not characterized.

Hemizygous: Transgenic mice have impaired insulin-signaling and insulin-stimulated glucose uptake into skeletal muscle, resulting in impaired whole-body glucose homeostasis.
MeSH Terms
  • Animals
  • Blood Glucose/metabolism
  • Body Composition
  • Creatine Kinase/genetics
  • Creatine Kinase, MM Form
  • Fatty Acids, Nonesterified/metabolism
  • Humans
  • Insulin/blood
  • Insulin/pharmacology
  • Insulin Resistance/genetics
  • Intracellular Signaling Peptides and Proteins
  • Isoenzymes/genetics
  • Mice
  • Mice, Transgenic
  • Muscles/drug effects
  • Muscles/enzymology
  • Muscles/metabolism
  • Organ Specificity
  • Phosphatidylinositol 3-Kinases/metabolism
  • Phosphorylation
  • Phosphotyrosine/metabolism
  • Promoter Regions, Genetic/genetics
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6
  • Protein Tyrosine Phosphatases/genetics
  • Protein Tyrosine Phosphatases/metabolism
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Signal Transduction/drug effects
  • Fatty Acids/metabolism
  • Genotype
  • Glucose/metabolism
  • HLA Antigens/metabolism
  • Insulin/metabolism
  • Insulin Resistance
  • Isoenzymes
  • Male
  • Phosphotyrosine/chemistry
  • Precipitin Tests
  • Protein Isoforms
  • Protein Kinase C/metabolism
  • Protein Tyrosine Phosphatase, Non-Receptor Type 1
  • Protein Tyrosine Phosphatases/physiology
  • Receptor, Insulin/metabolism
  • Signal Transduction
  • Transgenes
  • Tyrosine/chemistry
Strain Development
MCK-hLAR mice have been maintained by breeding heterozygous transgenic mice in donor's colony to FVB/NTac mice. On rare occasions when FVB/NTac mice were not available, FVB/NJ mice were used for breeding. For the last generation, sib matings from within the colony have been done.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Metabolism
  • Models for Human Disease
  • Research Tools
  • Skeletal muscle
Donor
Barbara B. Kahn, M.D. and Benjamin G. Neel, M.D., Ph.D., Beth Israel Deaconess Medical Center
Primary Reference
  • Zabolotny JM, Kim YB, Peroni OD, Kim JK, Pani MA, Boss O, Klaman LD, Kamatkar S, Shulman GI, Kahn BB, Neel BG. Overexpression of the LAR (leukocyte antigen-related) protein-tyrosine phosphatase in muscle causes insulin resistance. Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5187-92. Epub 2001 Apr 17. (Medline PMID: 11309481)
  • Zabolotny JM, Haj FG, Kim YB, Kim HJ, Shulman GI, Kim JK, Neel BG, Kahn BB. Transgenic overexpression of protein-tyrosine phosphatase 1B in muscle causes insulin resistance, but overexpression with leukocyte antigen-related phosphatase does not additively impair insulin action. J Biol Chem. 2004 Jun 4;279(23):24844-51. Epub 2004 Mar 18. (Medline PMID: 15031294)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
015507-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
015507-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
015507-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.