Strain Name:
B6.Cg-Tg(tetO-Creb1*)7922Nes/Mmmh
Stock Number:
017219-MU
Citation ID:
RRID:MMRRC_017219-MU

Strain Information

tetOp
Name: tetracycline operon
Type: Gene
Species: Synthetic
Chromosome:
Creb1
Name: cAMP responsive element binding protein 1
Type: Gene
Species: Rattus norvegicus (norway rat)
Chromosome: 9
Tg(tetO-Creb1*)7922Nes
Name: transgene insertion 7922, Eric Nestler
Synonyms: TetOp-mCREB line 4, TetOp-mCREB
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Plasmids driving rat mCREB alpha under control of TetOp were injected into pronuclei of oocytes from SJL/J X C57BL/6J mice.
Genotype Determination
Phenotype

Homozygous: Not characterized

Hemizygous: Phenotype of C57BL/6J mouse - no apparent phenotype

MeSH Terms
  • Anesthetics, Local/pharmacology
  • Animals
  • Bacterial Proteins/genetics
  • Bacterial Proteins/metabolism
  • Behavior, Addictive
  • Behavior, Animal
  • Cluster Analysis
  • Cocaine/pharmacology
  • Conditioning (Psychology)
  • Cyclic AMP Response Element-Binding Protein/genetics
  • Cyclic AMP Response Element-Binding Protein/metabolism
  • Gene Deletion
  • Gene Expression Regulation/drug effects
  • Gene Expression Regulation/genetics
  • Male
  • Mice
  • Mice, Transgenic
  • Nucleus Accumbens/drug effects
  • Nucleus Accumbens/metabolism
  • Oligonucleotide Array Sequence Analysis/methods
  • Phosphopyruvate Hydratase/genetics
  • Phosphopyruvate Hydratase/metabolism
  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-jun/genetics
  • RNA, Messenger/biosynthesis
  • RNA, Messenger/isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Reward
  • Time
  • Time Factors
Strain Development
Original founder (#7922) mated to C57BL/6J female. Subsequent generations mated to C57BL/6J mice, alternating males and females to not sex link gene. Donated to MMRRC on N16.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Neurobiology
  • Research Tools
  • Electrophysiology
Donor
Eric Nestler, M.D., Ph.D., University of Texas Southwestern Medical School
Primary Reference
McClung CA, Nestler EJ. Regulation of gene expression and cocaine reward by CREB and DeltaFosB. Nat Neurosci. 2003 Nov;6(11):1208-15. Epub 2003 Oct 19. (Medline PMID: 14566342)

Colony and Husbandry Information

No special requirements - they do like Nestlets or other nesting material.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
017219-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
017219-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
017219-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.